Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species - Archive ouverte HAL Access content directly
Journal Articles Journal of Infectious Diseases Year : 2022

Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species

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Abstract

We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.
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pasteur-03727545 , version 1 (19-07-2022)

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Attribution - NonCommercial - NoDerivatives - CC BY 4.0

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Camilla Luiza-Batista, Flore Nardella, Sabine Thiberge, Malika Serra-Hassoun, Marcelo U. Ferreira, et al.. Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species. Journal of Infectious Diseases, 2022, 225 (9), pp.1621-1625. ⟨10.1093/infdis/jiab431⟩. ⟨pasteur-03727545⟩
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