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Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species

Abstract : We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03727545
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Submitted on : Tuesday, July 19, 2022 - 1:01:50 PM
Last modification on : Wednesday, August 3, 2022 - 3:58:54 AM

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Camilla Luiza-Batista, Flore Nardella, Sabine Thiberge, Malika Serra-Hassoun, Marcelo U. Ferreira, et al.. Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species. Journal of Infectious Diseases, Oxford University Press (OUP), 2022, 225 (9), pp.1621-1625. ⟨10.1093/infdis/jiab431⟩. ⟨pasteur-03727545⟩

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