Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Journal of Infectious Diseases Année : 2022

Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species

Résumé

We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.
Fichier principal
Vignette du fichier
jiab431.pdf (3.58 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

pasteur-03727545 , version 1 (19-07-2022)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Camilla Luiza-Batista, Flore Nardella, Sabine Thiberge, Malika Serra-Hassoun, Marcelo U. Ferreira, et al.. Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species. Journal of Infectious Diseases, 2022, 225 (9), pp.1621-1625. ⟨10.1093/infdis/jiab431⟩. ⟨pasteur-03727545⟩
37 Consultations
56 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More