Generating High-Resolution Hi-C Contact Maps of Bacteria

Agnès Thierry (1) , Charlotte Cockram (1)

1 Régulation spatiale des Génomes - Spatial Regulation of Genomes

During the past decade, Chromosome Conformation Capture (3C/Hi-C)-based methods have been used to probe the 3D structure and organization of bacterial genomes, revealing fundamental aspects of chromosome dynamics. However, the current protocols are expensive, inefficient, and limited in their resolution. Here we present a simple, cost-effective Hi-C approach that is readily applicable to a range of Gram-positive and Gram-negative bacteria.

Mots clés: 3C; Bacteria; Chromosome conformation capture; Genome organization; Hi-C; Prokaryotes

Domaines: Sciences du Vivant [q-bio]

Agnès Thierry, Charlotte Cockram. Generating High-Resolution Hi-C Contact Maps of Bacteria. Hi-C Data Analysis, 2301, Springer US, pp.183-195, 2021, Methods in Molecular Biology, 978-1-0716-1390-0. ⟨10.1007/978-1-0716-1390-0_9⟩. ⟨pasteur-03686079⟩