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Journal articles
A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva
Abstract : Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm 2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.
https://hal-pasteur.archives-ouvertes.fr/pasteur-03323242
Contributor : Florence Jeannot Connect in order to contact the contributor
Submitted on : Friday, August 20, 2021 - 4:01:17 PM
Last modification on : Tuesday, January 4, 2022 - 11:56:02 AM
Long-term archiving on: : Sunday, November 21, 2021 - 9:01:13 PM
Contributor : Florence Jeannot Connect in order to contact the contributor
Submitted on : Friday, August 20, 2021 - 4:01:17 PM
Last modification on : Tuesday, January 4, 2022 - 11:56:02 AM
Long-term archiving on: : Sunday, November 21, 2021 - 9:01:13 PM
File
journal.pone.0249471.pdf
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Distributed under a Creative Commons Attribution 4.0 International License