A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva

Lisa Fourniol (1) , Yoann Madec (2) , Laurence Mousson (1) , Marie Vazeille (1) , Anna-Bella Failloux (1)

1 Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors
2 Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology

Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm 2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.

Domains: Life Sciences [q-bio]

Lisa Fourniol, Yoann Madec, Laurence Mousson, Marie Vazeille, Anna-Bella Failloux. A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva. PLoS ONE, 2021, 16 (4), pp.e0249471. ⟨10.1371/journal.pone.0249471⟩. ⟨pasteur-03323242⟩