A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2021

A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva

Résumé

Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm 2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.
Fichier principal
Vignette du fichier
journal.pone.0249471.pdf (1.09 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

pasteur-03323242 , version 1 (20-08-2021)

Licence

Paternité

Identifiants

Citer

Lisa Fourniol, Yoann Madec, Laurence Mousson, Marie Vazeille, Anna-Bella Failloux. A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva. PLoS ONE, 2021, 16 (4), pp.e0249471. ⟨10.1371/journal.pone.0249471⟩. ⟨pasteur-03323242⟩
32 Consultations
53 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More