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Book Sections Year : 2015

In Vivo Protein Cross-Linking

Abstract

In the cell, homo- and hetero-associations of polypeptide chains evolve and take place within subcellular compartments that are crowded with many other cellular macromolecules. In vivo chemical cross-linking of proteins is a powerful method to examine changes in protein oligomerization and protein-protein interactions upon cellular events such as signal transduction. This chapter is intended to provide a guide for the selection of cell membrane permeable cross-linkers, the optimization of in vivo cross-linking conditions, and the identification of specific cross-links in a cellular context where the frequency of random collisions is high. By combining the chemoselectivity of the homo-bifunctional cross-linker and the length of its spacer arm with knowledge on the protein structure, we show that selective cross-links can be introduced specifically on either the dimer or the hexamer form of the same polypeptide in vitro as well as in vivo, using the human type B nucleoside diphosphate kinase as a protein model.
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Dates and versions

pasteur-03277116 , version 1 (02-07-2021)

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Fabrice Agou, Michel Véron. In Vivo Protein Cross-Linking. Protein-Protein Interactions. Methods in Molecular Biology, vol 1278, 1278, Humana Press, pp.391-405, 2015, 978-1-4939-2425-7. ⟨10.1007/978-1-4939-2425-7_26⟩. ⟨pasteur-03277116⟩
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