In Vivo Protein Cross-Linking

Fabrice Agou (1) , Michel Véron (2)

1 Signalisation et Pathogénèse
2 Biochimie Structurale et Cellulaire

In the cell, homo- and hetero-associations of polypeptide chains evolve and take place within subcellular compartments that are crowded with many other cellular macromolecules. In vivo chemical cross-linking of proteins is a powerful method to examine changes in protein oligomerization and protein-protein interactions upon cellular events such as signal transduction. This chapter is intended to provide a guide for the selection of cell membrane permeable cross-linkers, the optimization of in vivo cross-linking conditions, and the identification of specific cross-links in a cellular context where the frequency of random collisions is high. By combining the chemoselectivity of the homo-bifunctional cross-linker and the length of its spacer arm with knowledge on the protein structure, we show that selective cross-links can be introduced specifically on either the dimer or the hexamer form of the same polypeptide in vitro as well as in vivo, using the human type B nucleoside diphosphate kinase as a protein model.

Mots clés: In vivo cross-linking; Oligomerization; NDPK-B; Nucleoside nucleotide metabolism; Multifunctional enzyme; Cysteine-mediated cross-linking; Histidine kinase; DNA binding protein; Metastasis

Domaines: Sciences du Vivant [q-bio]

Fabrice Agou, Michel Véron. In Vivo Protein Cross-Linking. Protein-Protein Interactions. Methods in Molecular Biology, vol 1278, 1278, Humana Press, pp.391-405, 2015, 978-1-4939-2425-7. ⟨10.1007/978-1-4939-2425-7_26⟩. ⟨pasteur-03277116⟩