Complete Nucleotide Sequence of Two Multidrug-Resistant IncR Plasmids from Klebsiella pneumoniae

Fabrice Compain (1) , L. Frangeul (2) , Laurence Drieux (1, 3) , Charlotte Verdet (4) , Sylvain Brisse (5) , Guillaume Arlet (1, 4) , Dominique Decré (1, 4)

1 UPMC - Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie
2 Génopole
3 CHU Pitié-Salpêtrière [AP-HP]
4 AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP)
5 Génomique évolutive des Microbes / Microbial Evolutionary Genomics

We report here the complete nucleotide sequence of two IncR replicons encoding multidrug resistance determinants, including β-lactam ( bla DHA-1 , bla SHV-12 ), aminoglycoside ( aphA1 , strA , strB ), and fluoroquinolone ( qnrB4 , aac6′-1b-cr ) resistance genes. The plasmids have backbones that are similar to each other, including the replication and stability systems, and contain a wide variety of transposable elements carrying known antibiotic resistance genes. This study confirms the increasing clinical importance of IncR replicons as resistance gene carriers.

Domaines: Bio-informatique [q-bio.QM]; Génomique, Transcriptomique et Protéomique [q-bio.GN]; Virologie

Fabrice Compain, L. Frangeul, Laurence Drieux, Charlotte Verdet, Sylvain Brisse, et al.. Complete Nucleotide Sequence of Two Multidrug-Resistant IncR Plasmids from Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy, 2014, 58 (7), pp.4207-4210. ⟨10.1128/AAC.02773-13⟩. ⟨pasteur-03252020⟩