Functional interaction between PML and SATB1 regulates chromatin-loop architecture and transcription of the MHC class I locus

Pavan Kumar P. (1, 2) , Oliver Bischof (2) , Prabhat Kumar Purbey (1) , Dimple Notani (1) , Henning Urlaub (3) , Anne Dejean (2) , Sanjeev Galande (1)

1 NCCS - National Centre for Cell Science [Pune, India]
2 Organisation Nucléaire et Oncogenèse
3 Max-Planck-Institut für Biophysikalische Chemie - Max Planck Institute for Biophysical Chemistry [Göttingen]

The function of the subnuclear structure the promyelocytic leukaemia (PML) body is unclear largely because of the functional heterogeneity of its constituents. Here, we provide the evidence for a direct link between PML, higher-order chromatin organization and gene regulation. We show that PML physically and functionally interacts with the matrix attachment region (MAR)-binding protein, special AT-rich sequence binding protein 1 (SATB1) to organize the major histocompatibility complex (MHC) class I locus into distinct higher-order chromatin-loop structures. Interferon gamma (IFNgamma) treatment and silencing of either SATB1 or PML dynamically alter chromatin architecture, thus affecting the expression profile of a subset of MHC class I genes. Our studies identify PML and SATB1 as a regulatory complex that governs transcription by orchestrating dynamic chromatin-loop architecture.

Domaines: Sciences du Vivant [q-bio]

Pavan Kumar P., Oliver Bischof, Prabhat Kumar Purbey, Dimple Notani, Henning Urlaub, et al.. Functional interaction between PML and SATB1 regulates chromatin-loop architecture and transcription of the MHC class I locus. Nature Cell Biology, 2006, 9 (1), pp.45-56. ⟨10.1038/ncb1516⟩. ⟨pasteur-03236983⟩