Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

Marie Desnos-Ollivier (1, 2) , Catherine Blanc (1, 2) , Dea Garcia-Hermoso (1, 2) , Damien Hoinard (1, 2) , Alexandre Alanio (3, 1, 2, 4) , Françoise Dromer (1, 2)

1 Mycologie moléculaire
2 CNRMA - Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire
3 UPD7 - Université Paris Diderot - Paris 7
4 Laboratoire de Parasitologie-Mycologie [CHU Saint Louis, Paris]

Saprochaete clavata and Magnusiomyces capitatus are human pathogens that are frequently mistaken for each other due to their similar phenotypes and erroneous or limited databases. Based on internal transcribed spacer (ITS) sequences, we propose species-specific carbon assimilation patterns and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) fingerprints that enable the identification of S. clavata, M. capitatus, and Galactomyces candidus to the species level.

Mots clés: Carbon; Cluster Analysis; Diagnostic Tests; Routine; DNA; Fungal; Ribosomal Spacer; Humans; Molecular Sequence Data; Mycological Typing Techniques; Mycoses; Phylogeny; Saccharomycetales; Sequence Analysis; Spectrometry; Mass; Matrix-Assisted Laser Desorption-Ionization

Domaines: Sciences du Vivant [q-bio]; Mycologie

Marie Desnos-Ollivier, Catherine Blanc, Dea Garcia-Hermoso, Damien Hoinard, Alexandre Alanio, et al.. Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases. Journal of Clinical Microbiology, 2014, 52 (6), pp.2196--2198. ⟨10.1128/JCM.00039-14⟩. ⟨pasteur-01405243⟩