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Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

Abstract : Saprochaete clavata and Magnusiomyces capitatus are human pathogens that are frequently mistaken for each other due to their similar phenotypes and erroneous or limited databases. Based on internal transcribed spacer (ITS) sequences, we propose species-specific carbon assimilation patterns and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) fingerprints that enable the identification of S. clavata, M. capitatus, and Galactomyces candidus to the species level.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01405243
Contributor : Floran Bidault <>
Submitted on : Tuesday, November 29, 2016 - 4:26:26 PM
Last modification on : Wednesday, October 14, 2020 - 4:17:14 AM

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Marie Desnos-Ollivier, Catherine Blanc, Dea Garcia-Hermoso, Damien Hoinard, Alexandre Alanio, et al.. Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases. Journal of Clinical Microbiology, American Society for Microbiology, 2014, 52 (6), pp.2196--2198. ⟨10.1128/JCM.00039-14⟩. ⟨pasteur-01405243⟩

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