Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

Marie Desnos-Ollivier; Catherine Blanc; Dea Garcia-Hermoso; Damien Hoinard; Alexandre Alanio; Françoise Dromer

doi:10.1128/JCM.00039-14

Journal articles

Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

Marie Desnos-Ollivier ^{1, 2} Catherine Blanc ^{1, 2} Dea Garcia-Hermoso ^{1, 2} Damien Hoinard ^{1, 2} Alexandre Alanio ^{3, 1, 2, 4} Françoise Dromer ^{1, 2}

1 Mycologie moléculaire

2 CNRMA - Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire

3 UPD7 - Université Paris Diderot - Paris 7

4 Laboratoire de Parasitologie-Mycologie [CHU Saint Louis, Paris]

Abstract : Saprochaete clavata and Magnusiomyces capitatus are human pathogens that are frequently mistaken for each other due to their similar phenotypes and erroneous or limited databases. Based on internal transcribed spacer (ITS) sequences, we propose species-specific carbon assimilation patterns and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) fingerprints that enable the identification of S. clavata, M. capitatus, and Galactomyces candidus to the species level.

Keywords : Carbon Cluster Analysis Diagnostic Tests Routine DNA Fungal Ribosomal Spacer Humans Molecular Sequence Data Mycological Typing Techniques Mycoses Phylogeny Saccharomycetales Sequence Analysis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

Life Sciences [q-bio] / Microbiology and Parasitology / Mycology

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Marie Desnos-Ollivier, Catherine Blanc, Dea Garcia-Hermoso, Damien Hoinard, Alexandre Alanio, et al.. Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases. Journal of Clinical Microbiology, American Society for Microbiology, 2014, 52 (6), pp.2196--2198. ⟨10.1128/JCM.00039-14⟩. ⟨pasteur-01405243⟩

Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

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Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

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