Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

Marie Desnos-Ollivier; Catherine Blanc; Dea Garcia-Hermoso; Damien Hoinard; Alexandre Alanio; Françoise Dromer

doi:10.1128/JCM.00039-14

Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

Marie Desnos-Ollivier ^{1, 2} Catherine Blanc ^{1, 2} Dea Garcia-Hermoso ^{1, 2} Damien Hoinard ^{1, 2} Alexandre Alanio ^{3, 1, 2, 4} Françoise Dromer ^{1, 2}

1 Mycologie moléculaire

2 CNRMA - Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire

3 UPD7 - Université Paris Diderot - Paris 7

4 Laboratoire de Parasitologie-Mycologie [Paris]

Abstract : Saprochaete clavata and Magnusiomyces capitatus are human pathogens that are frequently mistaken for each other due to their similar phenotypes and erroneous or limited databases. Based on internal transcribed spacer (ITS) sequences, we propose species-specific carbon assimilation patterns and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) fingerprints that enable the identification of S. clavata, M. capitatus, and Galactomyces candidus to the species level.

Keywords : Phylogeny Ribosomal Spacer Humans Molecular Sequence Data Mycological Typing Techniques Mycoses Carbon Cluster Analysis Diagnostic Tests Routine DNA Fungal Saccharomycetales Sequence Analysis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization

Type de document :

Article dans une revue

Journal of Clinical Microbiology, American Society for Microbiology, 2014, 52 (6), pp.2196--2198. 〈10.1128/JCM.00039-14〉

Domaine :

Sciences du Vivant [q-bio]

Sciences du Vivant [q-bio] / Microbiologie et Parasitologie / Mycologie

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https://hal-pasteur.archives-ouvertes.fr/pasteur-01405243
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Soumis le : mardi 29 novembre 2016 - 16:26:26
Dernière modification le : jeudi 11 janvier 2018 - 06:21:37

Marie Desnos-Ollivier, Catherine Blanc, Dea Garcia-Hermoso, Damien Hoinard, Alexandre Alanio, et al.. Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases. Journal of Clinical Microbiology, American Society for Microbiology, 2014, 52 (6), pp.2196--2198. 〈10.1128/JCM.00039-14〉. 〈pasteur-01405243〉

Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

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Misidentification of Saprochaete clavata as Magnusiomyces capitatus in clinical isolates: utility of internal transcribed spacer sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry and importance of reliable databases

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