Evolution and Population Structure of Salmonella enterica Serovar Newport

Vartul Sangal (1, 2) , Heather Harbottle (3) , Camila J Mazzoni (1, 4) , Reiner Helmuth (5) , Beatriz Guerra (5) , Xavier Didelot (6) , Bianca Paglietti (7) , Wolfgang Rabsch (8) , Sylvain Brisse (9) , François-Xavier Weill (10) , Philippe Roumagnac (1, 11) , Mark Achtman (1, 4)

1 Department of Molecular Biology [MPIIB Berlin]
2 Strathclyde Institute of Pharmacy and Biomedical Sciences
3 Center for Veterinary Medicine
4 Environmental Research Institute and Department of Microbiology
5 National Salmonella Reference Laboratory
6 Department of Statistics
7 Department of Biomedical Sciences
8 RKI - Robert Koch Institute [Berlin]
9 PF8 - Génotypage des Pathogènes et Santé Publique (Plate-forme)
10 CNR - Centre National de Référence des Salmonella - Bactéries pathogènes entériques
11 UMR BGPI - Biologie et Génétique des Interactions Plante-Parasite

Salmonellosis caused by Salmonella enterica serovar Newport is a major global public health concern, particularly because S. Newport isolates that are resistant to multiple drugs (MDR), including third-generation cephalosporins (MDR-AmpC phenotype), have been commonly isolated from food animals. We analyzed 384 S. Newport isolates from various sources by a multilocus sequence typing (MLST) scheme to study the evolution and population structure of the serovar. These were compared to the population structure of S. enterica serovars Enteritidis, Kentucky, Paratyphi B, and Typhimurium. Our S. Newport collection fell into three lineages, Newport-I, Newport-II, and Newport-III, each of which contained multiple sequence types (STs). Newport-I has only a few STs, unlike Newport-II or Newport-III, and has possibly emerged recently. Newport-I is more prevalent among humans in Europe than in North America, whereas Newport-II is preferentially associated with animals. Two STs of Newport-II encompassed all MDR-AmpC isolates, suggesting recent global spread after the acquisition of the bla CMY-2 gene. In contrast, most Newport-III isolates were from humans in North America and were pansusceptible to antibiotics. Newport was intermediate in population structure to the other serovars, which varied from a single monophyletic lineage in S. Enteritidis or S. Typhimurium to four discrete lineages within S. Paratyphi B. Both mutation and homologous recombination are responsible for diversification within each of these lineages, but the relative frequencies differed with the lineage. We conclude that serovars of S. enterica provide a variety of different population structures. Salmonellosis is a major global cause of diarrheal and extraintestinal disease in humans and animals (66). Salmonella enterica subspecies enterica (referred to herein as S. enterica) has been subdivided serologically into Ͼ1,500 se-rovars (35), but we focus on S. enterica serovar Newport (S. Newport) here because over the last decade it has been a very common cause of human salmonellosis in both the United States and Europe (13, 16, 27). Furthermore, mul-tidrug-resistant S. Newport isolates that are also resistant to extended-spectrum cephalosporins (MDR-AmpC) have now

Domaines: Santé publique et épidémiologie; Bactériologie

Vartul Sangal, Heather Harbottle, Camila J Mazzoni, Reiner Helmuth, Beatriz Guerra, et al.. Evolution and Population Structure of Salmonella enterica Serovar Newport. The Journal of applied bacteriology, 2010, 192 (24), pp.6465-75. ⟨10.1128/JB.00969-10⟩. ⟨pasteur-01133818⟩