1H NMR characterization of renatured lysozyme obtained from fully reduced lysozyme under folding/oxidation conditions: A high resolution liquid NMR study at magic angle spinning
Résumé
Magic angle spinning (MAS) has long been used as a powerful technique in studies of heterogeneous samples such as powdered solids, compartmentalized liquids and heterogeneous solid-liquid mixtures. Recently it has been shown that higher resolution could be achieved if high-resolution magnetic susceptibility matching probe technology was used in conjunction with MAS (Nano-probe). In this paper the possibilities and advantages of generating high-resolution spectra of liquids is illustrated using the Nano.nmr probe for the study in solution of lysozyme subjected to various treatments.