Solution structure of the orphan nuclear receptor rev-erb beta response element by 1H, 31P NMR and molecular simulation*.

Claire Castagné (1) , Henri Terenzi (2) , Mario M. Zakin (2) , Muriel Delepierre (1)

1 Résonance Magnétique Nucléaire
2 Expression des gènes eucaryotes

Rev-erb beta is an orphan receptor that binds as a homodimer or as a monomer to DNA. The solution structure of the non-palindromic 15 bp DNA duplex d(TAGAATGTAGGTCAG), the response element of Rev-erb beta for monomeric binding, was determined by 1H and 31P NMR, energy minimization with NMR-derived restraints for distances and NOE back-calculation methods. The refined final structures have the typical overall features of B-type DNA. However, titration of this 15 bp duplex with ReDBD, the DNA binding domain of Rev-erb beta, showed large shifts of imino protons and 31P signals, suggesting major conformational changes.

Domains: Structural Biology [q-bio.BM]

Claire Castagné, Henri Terenzi, Mario M. Zakin, Muriel Delepierre. Solution structure of the orphan nuclear receptor rev-erb beta response element by 1H, 31P NMR and molecular simulation*.. Biochimie, 2000, 82 (8), pp.739-48. ⟨10.1016/S0300-9084(00)01148-2⟩. ⟨pasteur-00369669⟩