Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs - Archive ouverte HAL Access content directly
Book Sections Year : 2019

Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs

(1) , (1) , (1) , (2)
1
2

Abstract

Chemical modification of nucleic acids can be achieved by the enzymatic polymerization of modified nucleoside triphosphates (dN*TPs). This approach obviates some of the requirements and drawbacks imposed by the more traditional solid-phase synthesis of oligonucleotides. Here, we describe the protocol that is necessary to synthesize dN*TPs and evaluate their substrate acceptance by polymerases for their subsequent use in various applications including selection experiments to identify aptamers. The protocol is exemplified for a sugar-constrained nucleoside analog, 7′,5′-bc-TTP.
Fichier principal
Vignette du fichier
Book chapter.pdf (667.02 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Licence : CC BY NC - Attribution - NonCommercial

Dates and versions

pasteur-03930944 , version 1 (09-01-2023)

Licence

Attribution - NonCommercial - CC BY 4.0

Identifiers

Cite

Stella Diafa, Damien Evéquoz, Christian Leumann, Marcel Hollenstein. Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs. Non-Natural Nucleic Acids, 1973, Humana, pp.1-13, 2019, Methods in Molecular Biology, ⟨10.1007/978-1-4939-9216-4_1⟩. ⟨pasteur-03930944⟩

Collections

PASTEUR CNRS
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More