Ruminant‐associated Listeria monocytogenes isolates belong preferentially to dairy‐associated hypervirulent clones: a longitudinal study in 19 farms - Archive ouverte HAL Access content directly
Journal Articles Environmental Microbiology Year : 2021

Ruminant‐associated Listeria monocytogenes isolates belong preferentially to dairy‐associated hypervirulent clones: a longitudinal study in 19 farms

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Juan J Quereda
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Abstract

Studies have shown that ruminants constitute reservoirs of Listeria monocytogenes, but little is known about the epidemiology and genetic diversity of this pathogen within farms. Here we conducted a large-scale longitudinal study to monitor Listeria spp. in 19 dairy farms during three consecutive seasons (N=3251 samples). L. innocua was the most prevalent species, followed by L. monocytogenes. L. monocytogenes was detected in 52.6% of farms and more frequently in cattle (4.1%) and sheep (4.5%) than in goat farms (0.2%). Lineage I accounted for 69% of L. monocytogenes isolates. Among animal samples, the most prevalent sublineages (SL) and clonal complexes (CC) were SL1/CC1, SL219/CC4, SL26/CC26 and SL87/CC87, whereas SL666/CC666 was most prevalent in environmental samples. 61 different L. monocytogenes cgMLST types were found, 28% common to different animals and/or surfaces within the same farm and 21% previously reported elsewhere in the context of food and human surveillance. L. monocytogenes prevalence was not affected by farm hygiene but by season: higher prevalence was observed during winter in cattle, and during winter and spring in sheep farms. Cows in their second lactation had a higher probability of L. monocytogenes fecal shedding. This study highlights dairy farms as a reservoir for hypervirulent L. monocytogenes.
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pasteur-03696055 , version 1 (15-06-2022)

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Carla Palacios-Gorba, Alexandra Moura, Jesús Gomis, Alexandre Leclercq, Ángel Gómez-Martín, et al.. Ruminant‐associated Listeria monocytogenes isolates belong preferentially to dairy‐associated hypervirulent clones: a longitudinal study in 19 farms. Environmental Microbiology, 2021, 23 (12), pp.7617-7631. ⟨10.1111/1462-2920.15860⟩. ⟨pasteur-03696055⟩
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