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Characterisation of the glnK-amtB operon and the involvement of AmtB in methylammonium uptake in Azorhizobium caulinodans

N. Michel-Reydellet 1, * N. Desnoues 1 M. de Zamaroczy 1 C. Elmerich 1 Pierre-Alexandre Kaminski 2
* Corresponding author
1 Physiologie Cellulaire
2 Biochimie cellulaire
Abstract : This work reports the characterisation of the Azorhizobium caulinodans amtB gene, the deduced protein sequence of which shares similarity to those of several ammonium transporters. amtB is located downstream from glnK, a glnB-like gene. It is cotranscribed with glnK from an NtrC- and sigma54-dependent promoter. glnK and amtB insertion mutant strains have been isolated. Methylammonium uptake was assayed in these strains and in other mutant strains in which the regulation of nitrogen metabolism is impaired. Our data suggest that the AmtB protein is an ammonium transporter, which is mainly regulated by NtrC in response to nitrogen availability.
Keywords : Nitrogen fixation Ammonium assimilation Ammonium transport
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Contributor : Pierre Alexandre Kaminski Connect in order to contact the contributor
Submitted on : Thursday, May 5, 2022 - 1:49:45 PM
Last modification on : Friday, May 6, 2022 - 3:38:37 AM

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PASTEUR | CNRS

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N. Michel-Reydellet, N. Desnoues, M. de Zamaroczy, C. Elmerich, Pierre-Alexandre Kaminski. Characterisation of the glnK-amtB operon and the involvement of AmtB in methylammonium uptake in Azorhizobium caulinodans. Molecular and General Genetics MGG, Springer Verlag, 1998, 258 (6), pp.671-677. ⟨10.1007/s004380050781⟩. ⟨pasteur-03659863⟩

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