HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

The gut environment regulates bacterial gene expression which modulates susceptibility to bacteriophage infection

Abstract : Abundance and diversity of bacteria and their viral predators, bacteriophages (phages), in the digestive tract are associated with human health. Particularly intriguing is the long-term coexistence of these two antagonistic populations. We performed genome-wide RNA sequencing on a human enteroaggregative Escherichia coli isolate to identify genes differentially expressed between in vitro conditions and in murine intestines. We experimentally demonstrated that four of these differentially expressed genes modified the interactions between E. coli and three virulent phages by either increasing or decreasing its susceptibility/resistance pattern and also by interfering with biofilm formation. Therefore, the regulation of bacterial genes expression during the colonization of the digestive tract influences the coexistence of phages and bacteria, highlighting the intricacy of tripartite relationships between phages, bacteria, and the animal host in intestinal homeostasis.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03644228
Contributor : Hugo Varet Connect in order to contact the contributor
Submitted on : Tuesday, April 19, 2022 - 8:42:17 AM
Last modification on : Wednesday, May 11, 2022 - 1:00:12 PM

Identifiers

Citation

Marta Lourenço, Lorenzo Chaffringeon, Quentin Lamy-Besnier, Marie Titécat, Thierry Pédron, et al.. The gut environment regulates bacterial gene expression which modulates susceptibility to bacteriophage infection. Cell Host & Microbe, 2022, 30 (4), pp.556-569.e5. ⟨10.1016/j.chom.2022.03.014⟩. ⟨pasteur-03644228⟩

Share

Metrics

Record views

16