The gut environment regulates bacterial gene expression which modulates susceptibility to bacteriophage infection

Marta Lourenço; Lorenzo Chaffringeon; Quentin Lamy-Besnier; Marie Titécat; Thierry Pédron; Odile Sismeiro; Rachel Legendre; Hugo Varet; Jean-Yves Coppée; Marion Bérard; Luisa de Sordi; Laurent Debarbieux

doi:10.1016/j.chom.2022.03.014

Journal articles

The gut environment regulates bacterial gene expression which modulates susceptibility to bacteriophage infection

Marta Lourenço ^{1, 2} Lorenzo Chaffringeon ^{1, 3, 4} Quentin Lamy-Besnier ¹ Marie Titécat ^{1, 5} Thierry Pédron ¹ Odile Sismeiro ^{6, 7} Rachel Legendre ^{6, 7, 8} Hugo Varet ^{6, 7, 8} Jean-Yves Coppée ^{6, 7} Marion Bérard ⁹ Luisa de Sordi ^{1, 3, 4} Laurent Debarbieux ^{1, *}

* Corresponding author

1 Bactériophage, bactérie, hôte - Bacteriophage, bacterium, host

Institut Pasteur [Paris], CNRS - Centre National de la Recherche Scientifique : UMR 6047, UPC - Université Paris Cité

2 Collège Doctoral

SU - Sorbonne Université

3 CRSA - Centre de Recherche Saint-Antoine

4 FHU PaCeMM - Fédération hospitalo-universitaire Paris Center for Microbiome Medicine

5 INFINITE (Ex-Liric) - Institute for Translational Research in Inflammation - U 1286

6 Biomics (plateforme technologique)

7 PF2 - Transcriptome et Epigénome

8 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB

9 C2RA - Animalerie Centrale (plateforme) - Central Animal Facility (platform)

Abstract : Abundance and diversity of bacteria and their viral predators, bacteriophages (phages), in the digestive tract are associated with human health. Particularly intriguing is the long-term coexistence of these two antagonistic populations. We performed genome-wide RNA sequencing on a human enteroaggregative Escherichia coli isolate to identify genes differentially expressed between in vitro conditions and in murine intestines. We experimentally demonstrated that four of these differentially expressed genes modified the interactions between E. coli and three virulent phages by either increasing or decreasing its susceptibility/resistance pattern and also by interfering with biofilm formation. Therefore, the regulation of bacterial genes expression during the colonization of the digestive tract influences the coexistence of phages and bacteria, highlighting the intricacy of tripartite relationships between phages, bacteria, and the animal host in intestinal homeostasis.

Keywords : auto-inducer biofilms enteric pathogens flagellum gene regulation intestinal microbiota lipopolysaccharide motility transcriptomics

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Journal articles

Domain :

Life Sciences [q-bio]

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https://hal-pasteur.archives-ouvertes.fr/pasteur-03644228
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Submitted on : Tuesday, April 19, 2022 - 8:42:17 AM
Last modification on : Wednesday, May 11, 2022 - 1:00:12 PM

The gut environment regulates bacterial gene expression which modulates susceptibility to bacteriophage infection

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The gut environment regulates bacterial gene expression which modulates susceptibility to bacteriophage infection

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