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The stress sigma factor σS/RpoS counteracts Fur repression of genes involved in iron and manganese metabolism and modulates the ionome of Salmonella enterica serovar Typhimurium

Abstract : In many Gram-negative bacteria, the stress sigma factor of RNA polymerase, σ S /RpoS, remodels global gene expression to reshape the physiology of quiescent cells and ensure their survival under non-optimal growth conditions. In the foodborne pathogen Salmonella enterica serovar Typhimurium, σ S is also required for biofilm formation and virulence. We have previously identified sRNAs genes positively controlled by σ S in Salmonella , including the two paralogous sRNA genes, ryhB1 and ryhB2 / isrE . Expression of ryhB1 and ryhB2 is repressed by the ferric uptake regulator Fur when iron is available. In this study, we show that σ S alleviates Fur-mediated repression of the ryhB genes and of additional Fur target genes. Moreover, σ S induces transcription of the manganese transporter genes mntH and sitABCD and prevents their repression, not only by Fur, but also by the manganese-responsive regulator MntR. These findings prompted us to evaluate the impact of a Δ rpoS mutation on the Salmonella ionome. Inductively coupled plasma mass spectrometry analyses revealed a significant effect of the Δ rpoS mutation on the cellular concentration of manganese, magnesium, cobalt and potassium. In addition, transcriptional fusions in several genes involved in the transport of these ions were regulated by σ S . This study suggests that σ S controls fluxes of ions that might be important for the fitness of quiescent cells. Consistent with this hypothesis, the Δ rpoS mutation extended the lag phase of Salmonella grown in rich medium supplemented with the metal ion chelator EDTA, and this effect was abolished when magnesium, but not manganese or iron, was added back. These findings unravel the importance of σ S and magnesium in the regrowth potential of quiescent cells.
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Selma Metaane, Véronique Monteil, Sophie Ayrault, Louise Bordier, Corinne Levi-Meyreuis, et al.. The stress sigma factor σS/RpoS counteracts Fur repression of genes involved in iron and manganese metabolism and modulates the ionome of Salmonella enterica serovar Typhimurium. PLoS ONE, Public Library of Science, 2022, 17 (3), pp.e0265511. ⟨10.1371/journal.pone.0265511⟩. ⟨pasteur-03634198⟩

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