Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples

Aline Rifflet (1) , Caroline Demangel (2) , Laure Guenin-Macé (2)

1 Biologie et Génétique de la Paroi bactérienne - Biology and Genetics of Bacterial Cell Wall
2 Immunobiologie de l'Infection - Immunobiology of Infection

Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.

Keywords: Mycobacterium ulcerans; LC-MS/MS; Folch’s extraction; Mycolactone; TLC

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Immunology

Aline Rifflet, Caroline Demangel, Laure Guenin-Macé. Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples. Mycobacterium ulcerans, 2387, Springer US, pp.117-130, 2021, Methods in Molecular Biology, 978-1-0716-1779-3. ⟨10.1007/978-1-0716-1779-3_13⟩. ⟨pasteur-03534701⟩