Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples - Institut Pasteur Access content directly
Book Sections Year : 2021

Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples

Abstract

Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.
Fichier principal
Vignette du fichier
pasteur-03534701.pdf (342.8 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

pasteur-03534701 , version 1 (31-01-2022)

Licence

Attribution - NonCommercial

Identifiers

Cite

Aline Rifflet, Caroline Demangel, Laure Guenin-Macé. Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples. Mycobacterium ulcerans, 2387, Springer US, pp.117-130, 2021, Methods in Molecular Biology, 978-1-0716-1779-3. ⟨10.1007/978-1-0716-1779-3_13⟩. ⟨pasteur-03534701⟩
34 View
87 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More