Harnessing MalE for the study of antigen/antibody recognitions

Hugues Bedouelle (1, 2) , Martial Renard (1, 2) , Laurent Belkadi (1, 2) , Patrick England (1, 2)

1 Département de Biologie structurale et Chimie - Department of Structural Biology and Chemistry
2 CNRS - Centre National de la Recherche Scientifique

The construction of hybrids between the variable fragment (Fv) of antibodies and protein MalE of Escherichia coli at the genetic level makes possible their preparation in a functional state, independently of any interaction with the antigen. We used such hybrids and a mutagenesis approach to study the recognition between antibody D1.3 and its antigen lysozyme, and its maturation. We subsequently transformed D1.3 into a reagentless fluorescent biosensor by knowledge-based design.

Keywords: Affinity; Amylose; Antibody; Antigen; Biosensor; Fluorescence; MalE; Maltose binding protein; Maturation; Purification; Recognition; Variable fragment

Domains: Life Sciences [q-bio]

Hugues Bedouelle, Martial Renard, Laurent Belkadi, Patrick England. Harnessing MalE for the study of antigen/antibody recognitions. Research in Microbiology, 2002, 153 (7), pp.395-398. ⟨10.1016/s0923-2508(02)01337-2⟩. ⟨pasteur-03525666⟩