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Harnessing MalE for the study of antigen/antibody recognitions

Abstract : The construction of hybrids between the variable fragment (Fv) of antibodies and protein MalE of Escherichia coli at the genetic level makes possible their preparation in a functional state, independently of any interaction with the antigen. We used such hybrids and a mutagenesis approach to study the recognition between antibody D1.3 and its antigen lysozyme, and its maturation. We subsequently transformed D1.3 into a reagentless fluorescent biosensor by knowledge-based design.
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Submitted on : Friday, January 14, 2022 - 6:18:03 AM
Last modification on : Thursday, April 7, 2022 - 10:10:53 AM

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Hugues Bedouelle, Martial Renard, Laurent Belkadi, Patrick England. Harnessing MalE for the study of antigen/antibody recognitions. Research in Microbiology, Elsevier, 2002, 153 (7), pp.395-398. ⟨10.1016/s0923-2508(02)01337-2⟩. ⟨pasteur-03525666⟩

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