HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Crystallization and preliminary X-ray analysis of aD-Ala:D-Ser ligase associated with VanG-type vancomycin resistance

Abstract : Acquired VanG-type resistance to vancomycin in Enterococcus faecalis BM4518 arises from inducible synthesis of peptidoglycan precursors ending in d-alanyld-serine, to which vancomycin exhibits low binding affinity. VanG, a d-alanine: d-serine ligase, catalyzes the ATP-dependent synthesis of the d-Ala-d-Ser dipeptide, which is incorporated into the peptidoglycan synthesis of VanG-type vancomycin-resistant strains. Here, the purification, crystallization and preliminary crystallographic analysis of VanG in complex with ADP are reported. The crystal belonged to space group P3 1 21, with unit-cell parameters a = b = 116.1, c = 177.2 Å , and contained two molecules in the asymmetric unit. A complete data set has been collected to 2.35 Å resolution from a single crystal under cryogenic conditions using synchrotron radiation.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03525336
Contributor : Hélène Ribierre Connect in order to contact the contributor
Submitted on : Thursday, January 13, 2022 - 5:46:22 PM
Last modification on : Thursday, April 7, 2022 - 10:10:29 AM
Long-term archiving on: : Thursday, April 14, 2022 - 11:37:45 PM

File

f-65-01024.pdf
Publisher files allowed on an open archive

Licence


Copyright

Identifiers

Collections

Citation

Patrick Weber, Djalal Meziane-Cherif, Ahmed Haouz, Frederick Saul, Patrice Courvalin. Crystallization and preliminary X-ray analysis of aD-Ala:D-Ser ligase associated with VanG-type vancomycin resistance. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, International Union of Crystallography, 2009, 65 (10), pp.1024 - 1026. ⟨10.1107/s1744309109033831⟩. ⟨pasteur-03525336⟩

Share

Metrics

Record views

7

Files downloads

8