Skip to Main content Skip to Navigation
New interface
Journal articles

Efficient Deamination of 5-Methylcytidine and 5-Substituted Cytidine Residues in DNA by Human APOBEC3A Cytidine Deaminase

Abstract : Deamination of 5-methylcytidine (5MeC) in DNA results in a G:T mismatch unlike cytidine (C) deamination which gives rise to a G:U pair. Deamination of C was generally considered to arise spontaneously. It is now clear that human APOBEC3A (A3A), a polynucleotide cytidine deaminase (PCD) with specificity for single stranded DNA, can extensively deaminate human nuclear DNA. It is shown here that A3A among all human PCDs can deaminate 5-methylcytidine in a variety of single stranded DNA substrates both in vitro and in transfected cells almost as efficiently as cytidine itself. This ability of A3A to accommodate 5-methyl moiety extends to other small and physiologically relevant substituted cytidine bases such as 5-hydroxy and 5-bromocytidine. As 5MeCpG deamination hotspots characterize many genes associated with cancer it is plausible that A3A is a major player in the onset of cancer.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03520095
Contributor : Jean-Pierre Vartanian Connect in order to contact the contributor
Submitted on : Monday, January 10, 2022 - 6:51:44 PM
Last modification on : Thursday, April 7, 2022 - 10:10:29 AM
Long-term archiving on: : Tuesday, April 12, 2022 - 12:51:46 AM

File

document(1).pdf
Publication funded by an institution

Identifiers

Collections

Citation

Rodolphe Suspène, Marie-Ming Aynaud, Jean Pierre Vartanian, Simon Wain-Hobson. Efficient Deamination of 5-Methylcytidine and 5-Substituted Cytidine Residues in DNA by Human APOBEC3A Cytidine Deaminase. PLoS ONE, 2013, 8 (6), pp.e63461. ⟨10.1371/journal.pone.0063461⟩. ⟨pasteur-03520095⟩

Share

Metrics

Record views

12

Files downloads

16