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Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node

Abstract : Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of lymph node T cells during either priming or tolerance, in live, anesthetized mice. Intravital imaging of T lymphocytes is a powerful tool to study the cellular orchestration of adaptive immune responses in physiological settings. This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03517264
Contributor : Susanna Celli Connect in order to contact the contributor
Submitted on : Friday, January 7, 2022 - 4:39:56 PM
Last modification on : Wednesday, January 12, 2022 - 8:18:01 PM

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Susanna Celli, Philippe Bousso. Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node. Fairchild, Paul J. Immunological Tolerance, 380, Humana Press, pp.355-363, 2007, Methods in Molecular Biology, 978-1-59745-395-0 (e-book). ⟨10.1007/978-1-59745-395-0_22⟩. ⟨pasteur-03517264⟩

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