Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node

Susanna Celli (1) , Philippe Bousso (1)

1 Département d'Immunologie - Department of Immunology

Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of lymph node T cells during either priming or tolerance, in live, anesthetized mice. Intravital imaging of T lymphocytes is a powerful tool to study the cellular orchestration of adaptive immune responses in physiological settings. This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts.

Keywords: T-cell activation; tolerance; lymph node; intravital imaging; two-photon microscopy

Domains: Immunology

Susanna Celli, Philippe Bousso. Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node. Fairchild, Paul J. Immunological Tolerance, 380, Humana Press, pp.355-363, 2007, Methods in Molecular Biology, 978-1-59745-395-0 (e-book). ⟨10.1007/978-1-59745-395-0_22⟩. ⟨pasteur-03517264⟩