Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells

Richard Patryk Ngondo (1, 2) , Michel Cohen-Tannoudji (3, 4) , Constance Ciaudo (5)

1 UNISTRA - Université de Strasbourg
2 IBMP - Institut de biologie moléculaire des plantes
3 IP - Institut Pasteur [Paris]
4 Embryon précoce de mammifères et cellules souches
5 ETH Zürich - Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich]

Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar molecular markers to the definitive endoderm. Here, we present a fast procedure to identify a differentiation defect of mESCs toward ExEn in vitro through the molecular and cellular characterization of embryoid bodies, followed by direct differentiation of mESCs into ExEn. For complete details on the use and execution of this protocol, please refer to Ngondo et al. (2018).

Domains: Development Biology

Richard Patryk Ngondo, Michel Cohen-Tannoudji, Constance Ciaudo. Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells. STAR Protocols, 2020, 1 (3), pp.100127. ⟨10.1016/j.xpro.2020.100127⟩. ⟨pasteur-03395363⟩