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Journal Articles Cell Reports Year : 2020

Individual Control and Quantification of 3D Spheroids in a High-Density Microfluidic Droplet Array

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Abstract

As three-dimensional cell culture formats gain in popularity, there emerges a need for tools that produce vast amounts of data on individual cells within the spheroids or organoids. Here, we present a microfluidic platform that provides access to such data by parallelizing the manipulation of individual spheroids within anchored droplets. Different conditions can be applied in a single device by triggering the merging of new droplets with the spheroid-containing drops. This allows cell-cell interactions to be initiated for building microtissues, studying stem cells’ self-organization, or observing antagonistic interactions. It also allows the spheroids’ physical or chemical environment to be modulated, as we show by applying a drug over a large range of concentrations in a single parallelized experiment. This convergence of microfluidics and image acquisition leads to a data-driven approach that allows the heterogeneity of 3D culture behavior to be addressed across the scales, bridging single-cell measurements with population measurements.
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Origin : Publication funded by an institution

Dates and versions

pasteur-03328779 , version 1 (30-08-2021)

Licence

Attribution - NonCommercial - NoDerivatives - CC BY 4.0

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Raphaël F.-X. Tomasi, Sébastien Sart, Tiphaine Champetier, Charles Baroud. Individual Control and Quantification of 3D Spheroids in a High-Density Microfluidic Droplet Array. Cell Reports, 2020, 31 (8), pp.107670. ⟨10.1016/j.celrep.2020.107670⟩. ⟨pasteur-03328779⟩
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