Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus

Christine Henry (1) , Jizhou Li (1) , François Danion (2, 1) , Laura Alcazar-Fuoli (3) , Emilia Mellado (3) , Rémi Beau (1) , Grégory Jouvion (4) , Jean-Paul Latgé (1) , Thierry Fontaine (1)

1 Aspergillus
2 Centre d'infectiologie Necker-Pasteur [CHU Necker]
3 ISC - Instituto de Salud Carlos III [Madrid]
4 Neuropathologie expérimentale - Experimental neuropathology

The fungal cell wall is a complex and dynamic entity essential for the development of fungi. It allows fungal pathogens to survive environmental challenge posed by nutrient stress and host defenses, and it also is central to polarized growth. The cell wall is mainly composed of polysaccharides organized in a three-dimensional network. Aspergillus fumigatus produces a cell wall galactomannan whose biosynthetic pathway and biological functions remain poorly defined. Here, we described two new mannosyltransferases essential to the synthesis of the cell wall galactomannan. Their absence leads to a growth defect with misregulation of polarization and altered conidiation, with conidia which are bigger and more permeable than the conidia of the parental strain. This study showed that in spite of its low concentration in the cell wall, this polysaccharide is absolutely required for cell wall stability, for apical growth, and for the full virulence of A. fumigatus .

Mots clés: Aspergillus fumigatus; KTR; biosynthesis; cell wall; galactomannan; mannosyltransferase

Domaines: Mycologie

Christine Henry, Jizhou Li, François Danion, Laura Alcazar-Fuoli, Emilia Mellado, et al.. Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus. mBio, 2019, 10 (1), pp.e02647-18. ⟨10.1128/mBio.02647-18⟩. ⟨pasteur-03264608⟩