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Journal articles
ILC3s control splenic cDC homeostasis via lymphotoxin signaling
Matthias Vanderkerken
1, 2
Antonio Baptista
1, 2, *
Marco de Giovanni
3
Satoshi Fukuyama
4
Robin Browaeys
1, 2
Charlotte Scott
1, 2
Paula Norris
5
Gerard Eberl
6
James Di Santo
7
Eric Vivier
8, 9
Yvan Saeys
1, 2
Hamida Hammad
1, 2
Jason Cyster
3
Carl Ware
5
Alexei Tumanov
10
Carl de Trez
11
Bart Lambrecht
1, 2, 12, *
*
Corresponding author
Matthias Vanderkerken 1, 2
Author
Antonio P. Baptista 1, 2
Correspondent author
Marco de Giovanni 3
Author
Satoshi Fukuyama 4
Author
Robin Browaeys 1, 2
Author
Charlotte L. Scott 1, 2
Author
Paula S. Norris 5
Author
Gerard Eberl 6
Author IdHAL : gerard-eberl ORCID : https://orcid.org/0000-0002-1119-5638
Bart N. Lambrecht 1, 2, 12
Correspondent author
1
IRC - VIB-UGent Center for Inflammation Research [Gand, Belgique] (UGent-VIB Research Building FSVM, Technologiepark 71, 9052 GENT, BELGIUM - Belgium)
- VIB [Belgium] (VIB Headquarters, Rijvisschestraat 120, 9052 Gent, Belgium - Belgium)
2
UGENT - Ghent University [Belgium] (St. Pietersnieuwstraat 33, 9000 Gent, - Belgium)
3
UCSF - University of California [San Francisco] (505 Parnassus Ave, San Francisco, CA 94143 - United States)
- University of California (United States)
4
UTokyo - The University of Tokyo (7 Chome-3-1 Hongo, Bunkyo, Tokyo 113-8654, Japon - Japan)
5
Sanford Burnham Prebys Medical Discovery Institute (La Jolla, CA, 92037 - United States)
6
Microenvironnement et Immunité - Microenvironment and Immunity (Département d'Immunologie, 25-28 rue du docteur Roux, 75724 Paris Cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1224 (101, rue de Tolbiac, 75013 Paris - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
7
Immunité Innée - Innate Immunity (Département d'Immunologie, 25-28 rue du Docteur Roux, 75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1223 (101, rue de Tolbiac, 75013 Paris - France)
8
Innate Pharma (117, Avenue de Luminy - BP 30191 13276 Marseille Cedex 09 - France)
9
CIML - Centre d'Immunologie de Marseille - Luminy (Parc scientifique et technologique de Luminy - 163, avenue de Luminy - Case 906 - 13288 Marseille cedex 09 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : UMR_S 1104 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7280 (France)
- AMU - Aix Marseille Université : UM 2 (Aix-Marseille Université Jardins du Pharo 58 Boulevard Charles Livon 13284 Marseille cedex 7 - France)
10
University of Texas Health Science Center (7703 Floyd Curl Drive San Antonio, Texas 78229-3900 - United States)
- UTHealth - The University of Texas Health Science Center at Houston (7000 Fannin, Suite 1200 Houston, Texas 77030 - United States)
11
VUB - Vrije Universiteit Brussel (Pleinlaan 2, 1050 Brussel - Belgium)
12
Erasmus MC - Erasmus University Medical Center [Rotterdam] (Rotterdam - Netherlands)
Abstract : The spleen contains a myriad of conventional dendritic cell (cDC) subsets that protect against systemic pathogen dissemination by bridging antigen detection to the induction of adaptive immunity. How cDC subsets differentiate in the splenic environment is poorly understood. Here, we report that LTα 1 β 2-expressing Rorgt + ILC3s, together with B cells, control the splenic cDC niche size and the terminal differentiation of Sirpα + CD4 + Esam + cDC2s, independently of the microbiota and of bone marrow pre-cDC output. Whereas the size of the splenic cDC niche depended on lymphotoxin signaling only during a restricted time frame, the homeostasis of Sirpα + CD4 + Esam + cDC2s required continuous lymphotoxin input. This latter property made Sirpα + CD4 + Esam + cDC2s uniquely susceptible to pharmacological interventions with LTβR agonists and antagonists and to ILC reconstitution strategies. Together, our findings demonstrate that LTα 1 β 2-expressing Rorgt + ILC3s drive splenic cDC differentiation and highlight the critical role of ILC3s as perpetual regulators of lymphoid tissue homeostasis.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03258252
Contributor : Bérengère Hugot <>
Submitted on : Friday, June 11, 2021 - 2:08:54 PM
Last modification on : Tuesday, June 22, 2021 - 3:42:10 PM
Contributor : Bérengère Hugot <>
Submitted on : Friday, June 11, 2021 - 2:08:54 PM
Last modification on : Tuesday, June 22, 2021 - 3:42:10 PM
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jem_20190835.pdf
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Distributed under a Creative Commons Attribution - NonCommercial - ShareAlike 4.0 International License
Identifiers
- HAL Id : pasteur-03258252, version 1
- DOI : 10.1084/jem.20190835
- PUBMED : 33724364
- PUBMEDCENTRAL : PMC7970251
Citation
Matthias Vanderkerken, Antonio Baptista, Marco de Giovanni, Satoshi Fukuyama, Robin Browaeys, et al.. ILC3s control splenic cDC homeostasis via lymphotoxin signaling. Journal of Experimental Medicine, Rockefeller University Press, 2021, 218 (5), pp.e20190835. ⟨10.1084/jem.20190835⟩. ⟨pasteur-03258252⟩
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