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Specific hepatitis B virus integration in hepatocellular carcinoma DNA through a viral 11-base-pair direct repeat

Anne Dejean 1 Pierre Sonigo 1 Simon Wain-Hobson 1 Pierre Tiollais 1
1 Recombinaison et expression génétique
Institut Pasteur [Paris], INSERM - Institut National de la Santé et de la Recherche Médicale : U 163, CNRS - Centre National de la Recherche Scientifique : URA 271
Abstract : Integrated hepatitis B virus (HBV) DNA sequences have been cloned from cellular DNA of two human liver tumors. The structure of the clones was determined by restriction mapping, and the host-viral DNA junctions were sequenced. In each clone one junction mapped to within an 11-base-pair sequence, 5' T-T-C-A-C-C-T-C-T-G-C, which is directly repeated near the extremities of the cohesive-end region of the free viral genome. The two copies of this sequence are termed DR1 and DR2. While one clone carried a host-viral junction within DR1, the second one carried a host-viral junction within DR2. The first 1 or 2 base pairs of the repeat were deleted upon recombination with the host genome, leaving at the junctions a common 9-base-pair segment of HBV DNA, 5' C-A-C-C-T-C-T-G-C. The other two host-viral junctions mapped to the pre-S region and to the core region of the viral genome, showing no peculiar feature. These results show that HBV DNA can integrate via a specific viral DNA sequence.
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Submitted on : Thursday, May 27, 2021 - 9:24:23 AM
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Anne Dejean, Pierre Sonigo, Simon Wain-Hobson, Pierre Tiollais. Specific hepatitis B virus integration in hepatocellular carcinoma DNA through a viral 11-base-pair direct repeat. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 1984, 81 (17), pp.5350-5354. ⟨10.1073/pnas.81.17.5350⟩. ⟨pasteur-03238348⟩



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