HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Close correlation between β-catenin gene alterations and nuclear accumulation of the protein in human hepatocellular carcinomas

Abstract : Several lines of evidence indicate that beta-catenin acquires oncogenic activity when its intracellular concentration increases as a result of either mutation in the beta-catenin gene itself or inactivation of the adenomatous polyposis coli (APC) gene. In an attempt to elucidate the molecular mechanisms underlying hepatocellular carcinogenesis, we have studied the frequency of beta-catenin gene alterations in exon 3, a region known to represent a mutation hot spot, and its inappropriate protein expression by immunohistochemistry in 73 hepatocellular carcinomas (HCCs). The results were correlated with different clinical and pathological data, particularly with the presence or not of an associated cirrhosis. Fourteen (19%) HCCs showed beta-catenin gene alterations with missense mutations in nine cases and interstitial deletions in five cases. These genetic alterations were present in both cirrhotic and non-cirrhotic groups. By contrast, we did not find any beta-catenin gene alterations in the nine fibromellar carcinomas we examined. Nuclear accumulation of the protein was observed in 18 of them (25%). Remarkably, these included ten of the 14 tumors harboring somatic mutations in the beta-catenin gene (P < 0.001). Our results indicate that accumulation of beta-catenin resulting from genetic mutations is a frequent event in non-fibrolamellar type hepatocellular carcinoma. The close association between increased beta-catenin protein stability and mutation indicates that immunohistochemistry may be a powerful method for the detection of the mutated protein in future clinical practice.
Document type :
Journal articles
Complete list of metadata

Contributor : Samia Cheriet Rauline Connect in order to contact the contributor
Submitted on : Wednesday, May 26, 2021 - 3:41:14 PM
Last modification on : Thursday, April 7, 2022 - 10:10:45 AM

Links full text




Benoît Terris, Pascal Pineau, Laurence Bregeaud, Dominique Valla, Jacques Belghiti, et al.. Close correlation between β-catenin gene alterations and nuclear accumulation of the protein in human hepatocellular carcinomas. Oncogene, Nature Publishing Group, 1999, 18 (47), pp.6583-6588. ⟨10.1038/sj.onc.1203051⟩. ⟨pasteur-03237225⟩



Record views