Skip to Main content Skip to Navigation
Preprints, Working Papers, ...

HIV-induced membraneless organelles orchestrate post-nuclear entry steps

Abstract : Abstract HIV integration occurs in chromatin sites that favor the release of high levels of viral progeny, alternatively the virus is also able to coexist discreetly with the host. To uncover the HIV-1 DNA fate in the nuclear landscape, we directly tracked the viral DNA and the viral RNA by coupling HIV-1 ANCHOR technology with RNA FISH or MCP-MS2 RNA-tagging bacterial system. Our computational imaging analysis revealed that proviral forms are early located in proximity of the nuclear periphery of mitotic and non-mitotic cells. HIV-1 triggers the nuclear clustering of the host factor CPSF6, but yet its role is poorly understood. Our data show that CPSF6 clusters are part of HIV-induced membraneless organelles (HIV-1 MLOs). Interestingly, we observed that viral genomic RNAs and the integrase proteins are sequestered in CPSF6 clusters, in which the virus can reverse transcribe, while the late retrotranscribed DNA is excluded from these structures. HIV-1 MLOs are formed in the interchromatin space, indicating that they are not proviral sites, but orchestrate viral events prior to the integration step. We found transcription-competent proviruses localize outside HIV-1 MLOs, in LEDGF-abundant regions, known to be active chromatin sites. This study highlights single functional host-proviral complexes in their nuclear landscape, which is markedly restructured by HIV-1 to favor viral replication.
Document type :
Preprints, Working Papers, ...
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03214329
Contributor : Francesca Di Nunzio <>
Submitted on : Saturday, May 1, 2021 - 12:45:45 PM
Last modification on : Wednesday, June 2, 2021 - 4:26:14 PM

Licence


Copyright

Identifiers

Collections

Citation

Viviana Scoca, Marion Louveaux, Renaud Morin, Dmitry Ershov, Jean-Yves Tinevez, et al.. HIV-induced membraneless organelles orchestrate post-nuclear entry steps. 2021. ⟨pasteur-03214329⟩

Share

Metrics

Record views

27

Files downloads

44