Docosahexaenoic fatty acid-containing phospholipids affect plasma membrane susceptibility to disruption by bacterial toxin-induced macroapertures - Archive ouverte HAL Access content directly
Preprints, Working Papers, ... Year :

Docosahexaenoic fatty acid-containing phospholipids affect plasma membrane susceptibility to disruption by bacterial toxin-induced macroapertures

(1, 2) , (1) , (3) , (4) , (2, 5) , (2, 5) , (1) , (6) , (6) , (1) , (4) , (2) , (1)
1
2
3
4
5
6

Abstract

Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (PLs) in physiology. Here, we study the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) tunnels triggered by the RhoA GTPase inhibitory exotoxin C3 from Clostridium botulinum . Through lipidomic analyses, we show that primary human umbilical vein endothelial cells (HUVECs) subjected to DHA-diet undergo a 6-fold DHA-PLs enrichment in plasma membrane at the expense of monounsaturated OA-PLs. In contrast, OA-diet had almost no effect on PLs composition. Consequently, DHA treatment increases the nucleation rate of TEMs by 2-fold that we ascribe to a reduction of cell thickness. We reveal that the global transcellular area of cells remains conserved through a reduction of the width and lifetime of TEMs. Altogether, we reveal a homeostasis between plasma membrane DHA-PLs content and large-scale membrane dynamics.
Fichier principal
Vignette du fichier
2020.12.23.424114v1.full.pdf (10.27 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

pasteur-03133140 , version 1 (05-02-2021)

Licence

Copyright

Identifiers

Cite

Meng-Chen Tsai, Lucile Fleuriot, Sébastien Janel, David Gonzalez-Rodriguez, Camille Morel, et al.. Docosahexaenoic fatty acid-containing phospholipids affect plasma membrane susceptibility to disruption by bacterial toxin-induced macroapertures. 2021. ⟨pasteur-03133140⟩
63 View
73 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More