OXA-244-Producing Escherichia coli Isolates, a Challenge for Clinical Microbiology Laboratories

Yannick Hoyos-Mallecot; Thierry Naas; Rémy Bonnin; Rafael Patino; Philippe Glaser; Nicolas Fortineau; Laurent Dortet

doi:10.1128/AAC.00818-17

Journal articles

OXA-244-Producing Escherichia coli Isolates, a Challenge for Clinical Microbiology Laboratories

Yannick Hoyos-Mallecot ^{1, 2, 3} Thierry Naas ^{1, 4, 2, 3, *} Rémy Bonnin ^{1, 4, 2, 3} Rafael Patino ⁴ Philippe Glaser ⁴ Nicolas Fortineau ^{1, 4, 2, 3} Laurent Dortet ^{1, 4, 2, 3}

* Corresponding author

1 AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre)

2 LabEx LERMIT - Laboratoire d'Excellence en Recherche sur le Médicament et l'Innovation Thérapeutique [Châtenay-Malabry]

3 Centre National de Référence Associé de la Résistance aux Antibiotiques

4 EERA - Ecologie et Evolution de la Résistance aux Antibiotiques / Ecology and Evolution of Antibiotics Resistance

Abstract : OXA-244 is a single-point-mutant derivative of OXA-48 displaying reduced carbapenemase activity. Here, we report the microbiological features of seven OXA-244-producing Escherichia coli isolates. Only one isolate grew on ChromID Carba Smart medium (bioMérieux), but six of the seven isolates grew on ChromID extended-spectrum-β-lactamase (ESBL) medium (bioMérieux), as they coproduced an ESBL and/or a plasmid-encoded cephalosporinase. The production of a carbapenemase was detected in 57.1%, 71.4%, 71.4%, and 100% of the E. coli isolates using the Carba NP test, the Rapidec Carba NP test (bioMérieux), a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) hydrolysis assay (Bruker), and the OXA-48 K-SeT assay (Coris BioConcept), respectively. Our results indicate that OXA-244-producing E. coli isolates are difficult to detect, which may lead to their silent spread.

Keywords : OXA-244 OXA-48-like carbapenemase activity detection screening tests

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

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https://hal-pasteur.archives-ouvertes.fr/pasteur-03107799
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Submitted on : Tuesday, January 12, 2021 - 7:32:35 PM
Last modification on : Monday, January 18, 2021 - 12:04:57 PM

OXA-244-Producing Escherichia coli Isolates, a Challenge for Clinical Microbiology Laboratories

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OXA-244-Producing Escherichia coli Isolates, a Challenge for Clinical Microbiology Laboratories

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