Forward Genetics in African Trypanosomes

Sebastian Hutchinson (1) , Lucy Glover (2)

1 Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology
2 Biologie moléculaire des Trypanosomes - Trypanosome Molecular Biology

Forward genetic screens in Trypanosoma brucei have enabled researchers to move from a candidate-gene based approach to one where we are able to studying all genes required for a single process simultaneously. In this protocol, we describe how to generate RNAi library strains in bloodstream form trypanosomes, run a screen by selecting for drug resistance or using a reporter gene and process the high-throughput sequencing data for a genome scale RNAi library screen.

Keywords: Forward genetics; High-throughput sequencing; Phenotypic screen; RNAi; Trypanosoma brucei

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Microbiology and Parasitology; Parasitology; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Genomics [q-bio.GN]

Sebastian Hutchinson, Lucy Glover. Forward Genetics in African Trypanosomes. Trypanosomatids, 2116, pp.339-352, 2020, Methods in Molecular biology, 978-1-0716-0294-2 (online) ; 978-1-0716-0293-5 (print). ⟨10.1007/978-1-0716-0294-2_21⟩. ⟨pasteur-03107149⟩