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Book sections
Forward Genetics in African Trypanosomes
Abstract : Forward genetic screens in Trypanosoma brucei have enabled researchers to move from a candidate-gene based approach to one where we are able to studying all genes required for a single process simultaneously. In this protocol, we describe how to generate RNAi library strains in bloodstream form trypanosomes, run a screen by selecting for drug resistance or using a reporter gene and process the high-throughput sequencing data for a genome scale RNAi library screen.
https://hal-pasteur.archives-ouvertes.fr/pasteur-03107149
Contributor : Lucy Glover <>
Submitted on : Tuesday, January 12, 2021 - 1:30:24 PM
Last modification on : Wednesday, January 13, 2021 - 4:15:53 PM
Contributor : Lucy Glover <>
Submitted on : Tuesday, January 12, 2021 - 1:30:24 PM
Last modification on : Wednesday, January 13, 2021 - 4:15:53 PM