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Forward Genetics in African Trypanosomes

Sebastian Hutchinson 1 Lucy Glover 2, * 
* Corresponding author
1 Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology
2 Biologie moléculaire des Trypanosomes - Trypanosome Molecular Biology
Abstract : Forward genetic screens in Trypanosoma brucei have enabled researchers to move from a candidate-gene based approach to one where we are able to studying all genes required for a single process simultaneously. In this protocol, we describe how to generate RNAi library strains in bloodstream form trypanosomes, run a screen by selecting for drug resistance or using a reporter gene and process the high-throughput sequencing data for a genome scale RNAi library screen.
Keywords : Forward genetics High-throughput sequencing Phenotypic screen RNAi Trypanosoma brucei
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03107149
Contributor : Lucy Glover Connect in order to contact the contributor
Submitted on : Tuesday, January 12, 2021 - 1:30:24 PM
Last modification on : Thursday, April 7, 2022 - 10:10:54 AM

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Sebastian Hutchinson, Lucy Glover. Forward Genetics in African Trypanosomes. Trypanosomatids, 2116, pp.339-352, 2020, Methods in Molecular biology, 978-1-0716-0294-2 (online) ; 978-1-0716-0293-5 (print). ⟨10.1007/978-1-0716-0294-2_21⟩. ⟨pasteur-03107149⟩

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