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Feed-forward recruitment of electrical synapses enhances synchronous spiking in the mouse cerebellar cortex

Andreas Hoehne 1, 2 Maureen Mcfadden 1 David A. Digregorio 1
1 Dynamique des synapses et des circuits neuronaux - Synapse and circuit dynamics
Institut Pasteur [Paris], CNRS - Centre National de la Recherche Scientifique : UMR3571
2 ED 158 - 3C - Ecole doctorale Cerveau Cognition et Comportement [Paris]
ISIR - Institut des Systèmes Intelligents et Robotique
Abstract : In the cerebellar cortex, molecular layer interneurons use chemical and electrical synapses to form subnetworks that fine-tune the spiking output of the cerebellum. Although electrical synapses can entrain activity within neuronal assemblies, their role in feed-forward circuits is less well explored. By combining whole-cell patch-clamp and 2-photon laser scanning microscopy of basket cells (BCs), we found that classical excitatory postsynaptic currents (EPSCs) are followed by GABA A receptor-independent outward currents, reflecting the hyperpolarization component of spikelets (a synapse-evoked action potential passively propagating from electrically coupled neighbors). FF recruitment of the spikelet-mediated inhibition curtails the integration time window of concomitant excitatory postsynaptic potentials (EPSPs) and dampens their temporal integration. In contrast with GABAergic-mediated feed-forward inhibition, the depolarizing component of spikelets transiently increases the peak amplitude of EPSPs, and thus postsynaptic spiking probability. Therefore, spikelet transmission can propagate within the BC network to generate synchronous inhibition of Purkinje cells, which can entrain cerebellar output for driving temporally precise behaviors.
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Andreas Hoehne, Maureen Mcfadden, David A. Digregorio. Feed-forward recruitment of electrical synapses enhances synchronous spiking in the mouse cerebellar cortex. eLife, eLife Sciences Publication, 2020, 9, ⟨10.7554/eLife.57344⟩. ⟨pasteur-02957831⟩

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