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Microfabricated Device for High-Resolution Imaging of Preimplantation Embryos

Sandrine Vandormael-Pournin 1 Emmanuel Frachon 2 Samy Gobaa 2 Michel Cohen-Tannoudji 1, *
* Corresponding author
1 Embryon précoce de mammifères et cellules souches - Early Mammalian Development & Stem Cell Biology
EPIC - Epigénomique, Prolifération et Identité Cellulaire - Epigenomics, Proliferation and the Identity of Cells
2 Biomatériaux et Microfluidique (Plateforme) - Biomaterials and Microfluidics (Platform)
C2RT - Centre de Ressources et de Recherche Technologique - Center for Technological Resources and Research
Abstract : The mouse preimplantation embryo is an excellent system for studying how mammalian cells organize dynamically into increasingly complex structures. Accessible to experimental and genetic manipulations, its normal or perturbed development can be scrutinized ex vivo by real-time imaging from fertilization to late blastocyst stage. High-resolution imaging of multiple embryos at the same time can be compromised by embryos displacement during imaging. We have developed an inexpensive and easy-to-produce imaging device that facilitates greatly the imaging of preimplantation embryo. In this chapter, we describe the different steps of production and storage of the imaging device as well as its use for live imaging of mouse preimplantation embryos expressing fluorescent reporters from genetically modified alleles or after in vitro transcribed mRNA transfer by microinjection or electroporation.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02951134
Contributor : Michel Cohen-Tannoudji <>
Submitted on : Monday, September 28, 2020 - 3:02:58 PM
Last modification on : Wednesday, October 14, 2020 - 4:10:32 AM

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Sandrine Vandormael-Pournin, Emmanuel Frachon, Samy Gobaa, Michel Cohen-Tannoudji. Microfabricated Device for High-Resolution Imaging of Preimplantation Embryos. Katia Ancelin; Maud Borensztein. Epigenetic Reprogramming During Mouse Embryogenesis : methods and protocols, 2214, Humana, pp.11-30, 2021, Methods in Molecular Biology, 978-1-0716-0957-6 (Print) ; 978-1-0716-0958-3 (Online). ⟨10.1007/978-1-0716-0958-3_2⟩. ⟨pasteur-02951134⟩

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