Tumor immunosurveillance and immunotherapies: a fresh look from intravital imaging

Morgane Boulch (1, 2) , Capucine L. Grandjean (1) , Marine Cazaux (1, 3) , Philippe Bousso (1)

1 Dynamiques des Réponses immunes - Dynamics of Immune Responses
2 ENS de Lyon - École normale supérieure de Lyon
3 Université Paris Diderot, Sorbonne Paris Cité, Paris, France

Understanding complex interactions between the immune system and the tumor microenvironment is an essential step towards the rational development and optimization of immunotherapies. Several experimental approaches are available to tackle this complexity but most are not designed to address the dynamic features of immune reactions, including cell migration, cellular interactions, and transient signaling events. By providing a unique means to access these precious parameters, intravital imaging offers a fresh look at intratumoral immune responses at the single-cell level. Here, we discuss how in vivo imaging sheds light on fundamental aspects of tumor immunity and helps elucidate modes of action of immunotherapies. We conclude by discussing future developments that may consolidate the unique contribution of intravital imaging for our understanding of tumor immunity.

Keywords: Tumor immunity; immunosurveillance; immunotherapy; intravital imaging; two- photon microscopy; tumor immunity; two-photon microscopy

Domains: Immunology

Morgane Boulch, Capucine L. Grandjean, Marine Cazaux, Philippe Bousso. Tumor immunosurveillance and immunotherapies: a fresh look from intravital imaging. Trends in Immunology, 2019, 40 (11), pp.1022-1034. ⟨10.1016/j.it.2019.09.002⟩. ⟨pasteur-02944694⟩