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Mitochondrial Dynamics and Activity in Legionella-Infected Cells
Abstract : The study of Legionella pneumophila interactions with host mitochondria during infection has been historically limited by the techniques available to analyze and quantify mitochondrial dynamics and activity in living cells. Recently, new, powerful techniques such as high-content microscopy or mitochondrial respiration assays (Seahorse) have been developed to quantitatively analyze mitochondrial parameters. Here we present state-of-the-art methods adapted to analyze mitochondrial dynamics and activity during Legionella infection of living human primary macrophages.
Cited literature [37 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-02881368
Contributor : Isabelle Hoffmann Connect in order to contact the contributor
Submitted on : Thursday, June 25, 2020 - 4:05:32 PM
Last modification on : Wednesday, June 16, 2021 - 5:26:02 PM
Long-term archiving on: : Wednesday, September 23, 2020 - 5:09:27 PM
Contributor : Isabelle Hoffmann Connect in order to contact the contributor
Submitted on : Thursday, June 25, 2020 - 4:05:32 PM
Last modification on : Wednesday, June 16, 2021 - 5:26:02 PM
Long-term archiving on: : Wednesday, September 23, 2020 - 5:09:27 PM
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