Mitochondrial Dynamics and Activity in Legionella-Infected Cells

Ok-Ryul Song (1) , Priscille Brodin (1) , Carmen Buchrieser (2) , Pedro Escoll (2)

1 CIIL - Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204
2 Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria

The study of Legionella pneumophila interactions with host mitochondria during infection has been historically limited by the techniques available to analyze and quantify mitochondrial dynamics and activity in living cells. Recently, new, powerful techniques such as high-content microscopy or mitochondrial respiration assays (Seahorse) have been developed to quantitatively analyze mitochondrial parameters. Here we present state-of-the-art methods adapted to analyze mitochondrial dynamics and activity during Legionella infection of living human primary macrophages.

Keywords: Mitochondrial dynamics; Legionella pneumophila; High-content microscopy; Seahorse; Mitochondrial respiration assay; Mitochondrial fission; Mitochondrial morphology; MitoTracker dyes; Bioenergetics; Living-cell assays

Domains: Life Sciences [q-bio]; Cellular Biology

Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, Pedro Escoll. Mitochondrial Dynamics and Activity in Legionella-Infected Cells. Carmen Buchrieser; Hubert Hilbi. Legionella: Methods and Protocols, 1921 (13), Springer Science; Business Media, LLC, pp.205-220, 2019, Methods in Molecular Biology (MIMB), 978-1-4939-9047-4 (Print), 978-1-4939-9048-1 (Online). ⟨10.1007/978-1-4939-9048-1_13⟩. ⟨pasteur-02881368⟩