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Journal articles
Caspase-1 Activation of IL-1β and IL-18 Are Essential for Shigella flexneri–Induced Inflammation
Abstract : Caspases are intracellular proteases that mediate mammalian cell apoptosis. Caspase-1 (Casp-1) is a unique caspase because it activates the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Shigella flexneri, the etiological agent of bacillary dysentery, induces macrophage apoptosis, which requires Casp-1 and results in the release of mature IL-1beta and IL-18. Here we show that casp-1(-/-) mice infected with S. flexneri do not develop the acute inflammation characteristic of shigellosis and are unable to resolve the bacterial infection. Using casp-1(-/-) mice supplemented with recombinant cytokines and experiments with IL-1beta(-/-) and IL-18(-/-) mice, we show that IL-1beta and IL-18 are both required to mediate inflammation in S. flexneri infections. Together, these data demonstrate the importance of Casp-1 in acute inflammation and show the different roles of its substrates, IL-1beta and IL-18, in this response.
https://hal-pasteur.archives-ouvertes.fr/pasteur-02875041
Contributor : Armelle Phalipon Connect in order to contact the contributor
Submitted on : Friday, June 19, 2020 - 12:59:17 PM
Last modification on : Monday, January 3, 2022 - 4:36:02 PM
Contributor : Armelle Phalipon Connect in order to contact the contributor
Submitted on : Friday, June 19, 2020 - 12:59:17 PM
Last modification on : Monday, January 3, 2022 - 4:36:02 PM
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