Anti-Inflammatory Role for Intracellular Dimeric Immunoglobulin A by Neutralization of Lipopolysaccharide in Epithelial Cells

M.Isabel Fernandez (1) , Thierry Pedron (1) , Régis R. Tournebize (1) , Jean-Christophe Olivo-Marin (2) , Philippe Sansonetti (1) , Armelle Phalipon (1)

1 Pathogénie Microbienne Moléculaire
2 BIA - Analyse d'images biologiques - Biological Image Analysis

Intestinal epithelial cells (IEC) play a central role in innate and acquired mucosal immunity. They ensure early signaling to trigger an inflammatory response against pathogens. Moreover, IEC mediate transcytosis of dimeric IgA (dIgA), through the polymeric-immunoglobulin receptor (pIgR), to provide secretory IgA, the major protective Ig in mucosal secretions. Using an in vitro model of polarized IEC, we describe an additional anti-inflammatory mechanism of dIgA-mediated protection against intracellular bacterial components involved in the proinflammatory activation of IEC. Specific dIgA colocalizes to lipopolysaccharide (LPS) in the apical recycling endosome compartment, preventing LPS-induced NF-kappaB translocation and subsequent proinflammatory response. Thus, intracellular neutralization by dIgA limits the acute local inflammation induced by proinflammatory pathogen-associated molecular patterns such as LPS.

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

M.Isabel Fernandez, Thierry Pedron, Régis R. Tournebize, Jean-Christophe Olivo-Marin, Philippe Sansonetti, et al.. Anti-Inflammatory Role for Intracellular Dimeric Immunoglobulin A by Neutralization of Lipopolysaccharide in Epithelial Cells. Immunity, 2003, 18 (6), pp.739-749. ⟨10.1016/S1074-7613(03)00122-5⟩. ⟨pasteur-02875032⟩