Skip to Main content Skip to Navigation
Journal articles

SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States

Abstract : Understanding general principles that safeguard cellular identity should reveal critical insights into common mechanisms underlying specification of varied cell types. Here, we show that SUMO modification acts to stabilize cell fate in a variety of contexts. Hyposumoylation enhances pluripotency reprogramming in vitro and in vivo, increases lineage transdifferentiation, and facilitates leukemic cell differentiation. Suppressing sumoylation in embryonic stem cells (ESCs) promotes their conversion into 2-cell-embryo-like (2C-like) cells. During reprogramming to pluripotency, SUMO functions on fibroblastic enhancers to retain somatic transcription factors together with Oct4, Sox2, and Klf4, thus impeding somatic enhancer inactivation. In contrast, in ESCs, SUMO functions on heterochromatin to silence the 2C program, maintaining both proper H3K9me3 levels genome-wide and repression of the Dux locus by triggering recruitment of the sumoylated PRC1.6 and Kap/Setdb1 repressive complexes. Together, these studies show that SUMO acts on chromatin as a glue to stabilize key determinants of somatic and pluripotent states.
Document type :
Journal articles
Complete list of metadata

Cited literature [46 references]  Display  Hide  Download
Contributor : Jacob-Sebastian Seeler Connect in order to contact the contributor
Submitted on : Monday, June 15, 2020 - 6:35:42 PM
Last modification on : Friday, August 5, 2022 - 3:05:12 PM



Links full text



Jack-Christophe Cossec, Ilan Theurillat, Claudia Chica, Sabela Búa Aguín, Xavier Gaume, et al.. SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States. Cell Stem Cell, Cambridge, MA : Cell Press, 2018, 23 (5), pp.742-757.e8. ⟨10.1016/j.stem.2018.10.001⟩. ⟨pasteur-02869159⟩



Record views