Skip to Main content Skip to Navigation
Book sections

A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae

Abstract : Homologous recombination faithfully restores the sequence information interrupted by a DNA double-strand break by referencing an intact DNA molecule as a template for repair DNA synthesis. DNA synthesis is primed from 3'-OH end of the invading DNA strand in the displacement loop (D-loop). Here, we describe a simple and quantitative proximity ligation-based assay to study the initiation of homologous recombination-associated DNA synthesis initiated at the D-loop and final product formation. The D-loop extension assay overcomes the semiquantitative nature and some limitations of the current PCR-based technique and facilitates the study of the recombination-associated DNA synthesis.
Document type :
Book sections
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-02868112
Contributor : Romain Koszul <>
Submitted on : Monday, June 15, 2020 - 12:18:17 PM
Last modification on : Wednesday, June 17, 2020 - 3:27:11 AM

Links full text

Identifiers

Collections

Citation

Aurele Piazza, Romain Koszul, Wolf-Dietrich Heyer. A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae. Mechanisms of DNA Recombination and Genome Rearrangements: Intersection between Homologous Recombination, DNA Replication and DNA Repair, 601, pp.27-44, 2018, Methods in enzymology, 978-0-12-813979-0. ⟨10.1016/bs.mie.2017.11.024⟩. ⟨pasteur-02868112⟩

Share

Metrics

Record views

28