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A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae
Abstract : Homologous recombination faithfully restores the sequence information interrupted by a DNA double-strand break by referencing an intact DNA molecule as a template for repair DNA synthesis. DNA synthesis is primed from 3'-OH end of the invading DNA strand in the displacement loop (D-loop). Here, we describe a simple and quantitative proximity ligation-based assay to study the initiation of homologous recombination-associated DNA synthesis initiated at the D-loop and final product formation. The D-loop extension assay overcomes the semiquantitative nature and some limitations of the current PCR-based technique and facilitates the study of the recombination-associated DNA synthesis.
https://hal-pasteur.archives-ouvertes.fr/pasteur-02868112
Contributor : Romain Koszul Connect in order to contact the contributor
Submitted on : Monday, June 15, 2020 - 12:18:17 PM
Last modification on : Tuesday, November 23, 2021 - 2:38:07 PM
Contributor : Romain Koszul Connect in order to contact the contributor
Submitted on : Monday, June 15, 2020 - 12:18:17 PM
Last modification on : Tuesday, November 23, 2021 - 2:38:07 PM
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