Skip to Main content Skip to Navigation
Journal articles

Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb

Abstract : During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful framework to elucidate the mechanisms of morphogenesis. To study the cellular basis of limb development, we imaged transgenic fluorescently-labeled embryos from the crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days of embryogenesis. The cell lineage of outgrowing thoracic limbs was reconstructed at single-cell resolution with new software called Massive Multi-view Tracker (MaMuT). In silico clonal analyses suggested that the early limb primordium becomes subdivided into anterior-posterior and dorsal-ventral compartments whose boundaries intersect at the distal tip of the growing limb. Limb-bud formation is associated with spatial modulation of cell proliferation, while limb elongation is also driven by preferential orientation of cell divisions along the proximal-distal growth axis. Cellular reconstructions were predictive of the expression patterns of limb development genes including the BMP morphogen Decapentaplegic.
Document type :
Journal articles
Complete list of metadatas

Cited literature [112 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02616453
Contributor : Jean-Yves Tinevez <>
Submitted on : Sunday, May 24, 2020 - 6:44:10 PM
Last modification on : Friday, July 24, 2020 - 9:58:06 AM

File

Multi-view light-sheet imaging...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Carsten Wolff, Jean-Yves Tinevez, Tobias Pietzsch, Evangelia Stamataki, Benjamin Harich, et al.. Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb. eLife, eLife Sciences Publication, 2018, 7, pp.e34410. ⟨10.7554/eLife.34410⟩. ⟨pasteur-02616453⟩

Share

Metrics

Record views

53

Files downloads

138