Intracellular offspring released from SFB filaments are flagellated

Iris Nkamba (1, 2) , Céline Mulet (3) , Gyanendra Dubey (3) , Olivier Gorgette (4) , Aurélie Couesnon (1) , Audrey Salles (5) , Maryse Moya-Nilges (4) , Vincent Jung (6) , Valérie Gaboriau-Routhiau (1, 7) , Ida Chiara Guerrera (6) , Tatsuichiro Shima (8) , Yoshinori Umesaki (8) , Giulia S Nigro (3) , Jacomina Krijnse-Locker (4) , Marion Bérard (9) , Nadine Cerf-Bensussan (1, 2) , Philippe Sansonetti (3, 10) , Pamela Schnupf (1, 2, 11)

1 Equipe Inserm U1163 - Laboratory of Intestinal Immunity
2 UFR SHS UPCité - UFR Sciences humaines et sociales [Sociétés et Humanités] - Université Paris Cité
3 Pathogénie microbienne moléculaire
4 UTechS UBI - Plateforme BioImagerie Ultrastructurale – Ultrastructural BioImaging Platform
5 UTechS PBI - BioImagerie Photonique – Photonic BioImaging
6 PPN - 3P5 - Plateforme Protéomique Necker [SFR Necker]
7 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
8 Yakult Central institute [Tokyo]
9 Animalerie centrale (Plate-forme)
10 Collège de France - Chaire Microbiologie et Maladies infectieuses
11 Laboratoire Interaction Hôte-Microbiote [Institut Necker Enfants Malades, Paris]

The gut commensal segmented filamentous bacterium (SFB) attaches to the ileal epithelium and potently stimulates the host immune system. Using transmission electron microscopy (TEM), we show that mouse and rat SFB are flagellated above the concave tip at the unicellular intracellular offspring (IO) stage and that flagellation occurs prior to full IO differentiation and release of IOs from SFB filaments. This finding adds a missing link to the SFB life cycle.

Keywords: Bacterial host response; Bacterial physiology; Bacterial development

Domains: Life Sciences [q-bio]

Iris Nkamba, Céline Mulet, Gyanendra Dubey, Olivier Gorgette, Aurélie Couesnon, et al.. Intracellular offspring released from SFB filaments are flagellated. Nature Microbiology, 2020, 5 (1), pp.34-39. ⟨10.1038/s41564-019-0608-1⟩. ⟨pasteur-02557372⟩