Skip to Main content Skip to Navigation
Journal articles

Single-Cell Acoustic Force Spectroscopy (scAFS): Resolving kinetics and strength of T-cell adhesion to fibronectin

Abstract : Assessing strength and kinetics of molecular interactions of cells with the extracellular matrix is fundamental to understand cell-adhesion processes. Given the relevance of these processes, there is a strong need for physical methods to quantitatively assess the mechanism of cell adhesion at the single-cell level, allowing discrimination of cells with different behaviours. Here we introduce single-cell Acoustic Force Spectroscopy (scAFS), an approach that makes use of acoustic waves to exert controlled forces, up to 1 nN, to hundreds of individual cells in parallel. We demonstrate the potential of scAFS by measuring adhesion forces and kinetics of CD4 + T-lymphocytes (CD4) to fibronectin. We determined that CD4 adhesion is accelerated by interleukin-7, their main regulatory cytokine, while CD4 binding strength remains the same. Activation of these cells likely increases their chance to bind to the vessel wall in the blood flow to infiltrate inflamed tissues and locally coordinate the immune response.
Complete list of metadatas

Cited literature [36 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02361962
Contributor : Pascal Bochet <>
Submitted on : Wednesday, November 13, 2019 - 4:17:03 PM
Last modification on : Monday, May 18, 2020 - 11:00:36 AM

File

CELL-REPORTS-D-17-03731_Final_...
Files produced by the author(s)

Identifiers

Collections

Citation

Douwe Kamsma, Pascal Bochet, Felix Oswald, Nander Ablas, Sophie Goyard, et al.. Single-Cell Acoustic Force Spectroscopy (scAFS): Resolving kinetics and strength of T-cell adhesion to fibronectin. Cell Reports, Elsevier Inc, 2018, 24 (11), pp.3008-3016. ⟨10.1016/j.celrep.2018.08.034⟩. ⟨pasteur-02361962⟩

Share

Metrics

Record views

84

Files downloads

114