MALDI-TOF mass spectrometry-based identification of Listeria species in surveillance: a prospective study

Pierre Thouvenot (1) , Guillaume Vales (1) , Hélène Bracq-Dieye (1) , Nathalie Tessaud-Rita (1) , Mylène M Maury (1) , Alexandra Moura (1) , Marc Lecuit (2, 1, 3, 4, 5) , Alexandre Leclercq (1)

1 CC-OMS / WHO-CC - Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria
2 Biologie des Infections - Biology of Infection
3 UPD5 - Université Paris Descartes - Paris 5
4 CHU Necker - Enfants Malades [AP-HP]
5 Imagine - U1163 - Imagine - Institut des maladies génétiques (IHU)

This study aimed to evaluate MALDI-TOF MS for species discrimination of Listeria in the context of routine surveillance. MALDI-TOF MS yielded 100% accuracy for the identification of L. monocytogenes, L. innocua, L. ivanovii, L. fleischmannii, L. grayi, L. seeligeri, L. weihenstephanensis and L. welshimeri, as confirmed by whole genome sequence analyses.

Keywords: Food; Listeria identification; MALDI-TOF; Mass spectrometry; Public health surveillance

Domains: Life Sciences [q-bio]; Human health and pathology; Infectious diseases; Life Sciences [q-bio]; Microbiology and Parasitology; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Pierre Thouvenot, Guillaume Vales, Hélène Bracq-Dieye, Nathalie Tessaud-Rita, Mylène M Maury, et al.. MALDI-TOF mass spectrometry-based identification of Listeria species in surveillance: a prospective study. Journal of Microbiological Methods, 2017, 144, pp.29-32. ⟨10.1016/j.mimet.2017.10.009⟩. ⟨pasteur-02320046⟩