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MALDI-TOF mass spectrometry-based identification of Listeria species in surveillance: a prospective study

Pierre Thouvenot 1 Guillaume Vales 1 Hélène Bracq-Dieye 1 Nathalie Tessaud-Rita 1 Mylène Maury 1 Alexandra Moura 1 Marc Lecuit 2, 1, 3, 4, 5, * Alexandre Leclercq 1, *
* Corresponding author
1 CCOMS - Centre collaborateur de l'OMS Listeria - Biologie des Infections
2 Biologie des Infections - Biology of Infection
3 UPD5 - Université Paris Descartes - Paris 5
4 CHU Necker - Enfants Malades [AP-HP]
5 Imagine - U1163 - Imagine - Institut des maladies génétiques (IHU)
Abstract : This study aimed to evaluate MALDI-TOF MS for species discrimination of Listeria in the context of routine surveillance. MALDI-TOF MS yielded 100% accuracy for the identification of L. monocytogenes, L. innocua, L. ivanovii, L. fleischmannii, L. grayi, L. seeligeri, L. weihenstephanensis and L. welshimeri, as confirmed by whole genome sequence analyses.
Keywords : Food Listeria identification MALDI-TOF Mass spectrometry Public health surveillance
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PASTEUR | UNIV-PARIS5 | USPC | INSERM | UNIV-PARIS | POLITIQUES-PUBLIQUES | UP-SANTE

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Pierre Thouvenot, Guillaume Vales, Hélène Bracq-Dieye, Nathalie Tessaud-Rita, Mylène Maury, et al.. MALDI-TOF mass spectrometry-based identification of Listeria species in surveillance: a prospective study. Journal of Microbiological Methods, Elsevier, 2017, 144, pp.29-32. ⟨10.1016/j.mimet.2017.10.009⟩. ⟨pasteur-02320046⟩

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