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Journal articles
Target gene sequencing to define the susceptibility of Neisseria meningitidis to ciprofloxacin.
Eva Hong
1
Sara Thulin Hedberg
2
Raquel Abad
3
Cecilia Fazio
4
Rocío Enríquez
3
Ala-Eddine Deghmane
1
Keith Jolley
5
Paola Stefanelli
4
Magnus Unemo
2
Julio Vazquez
3
Frédéric Veyrier
1
Muhamed-Kheir Taha
1, *
*
Corresponding author
Rocío Enríquez 3
Author
Ala-Eddine Deghmane 1
Author IdHAL : ala-eddinedeghmane ORCID : https://orcid.org/0000-0003-2887-1732
Keith A. Jolley 5
Author
Paola Stefanelli 4
Author
Magnus Unemo 2
Author
Julio A Vazquez 3
Author
Frédéric Veyrier 1
Author
Muhamed-Kheir Taha 1
Correspondent author IdHAL : muhamed-kheir-taha ORCID : https://orcid.org/0000-0002-0716-3174
1
CNR - Centre National de Référence des Méningocoques et Haemophilus influenzae - National Reference Center Meningococci and Haemophilus influenzae (Unité Infections bactériennes invasives
25-28 rue du Docteur Roux - 75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
3
ISC - Instituto de Salud Carlos III [Madrid] ( Carretera de Majadahonda - Pozuelo, 28220 Majadahonda, Madrid, Espagne - Spain)
4
Department of Infectious, Parasitic and Immune-Mediated Diseases [Rome] (Viale Regina Elena 299;00161;Rome - Italy)
5
University of Oxford [Oxford] (Wellington Square, Oxford OX1 2JD - United Kingdom)
Abstract : Meningococcal gyrA gene sequence data, MICs, and mouse infection were used to define the ciprofloxacin breakpoint for Neisseria meningitidis. Residue T91 or D95 of GyrA was altered in all meningococcal isolates with MICs of ≥ 0.064 μg/ml but not among isolates with MICs of ≤ 0.032 μg/ml. Experimental infection of ciprofloxacin-treated mice showed slower bacterial clearance when GyrA was altered. These data suggest a MIC of ≥ 0.064 μg/ml as the ciprofloxacin breakpoint for meningococci and argue for the molecular detection of ciprofloxacin resistance.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02089320
Contributor : Marie Martin Connect in order to contact the contributor
Submitted on : Wednesday, April 3, 2019 - 4:09:34 PM
Last modification on : Friday, November 26, 2021 - 4:24:05 PM
Contributor : Marie Martin Connect in order to contact the contributor
Submitted on : Wednesday, April 3, 2019 - 4:09:34 PM
Last modification on : Friday, November 26, 2021 - 4:24:05 PM
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- HAL Id : pasteur-02089320, version 1
- DOI : 10.1128/AAC.02184-12
- PUBMED : 23357770
- PUBMEDCENTRAL : PMC3623314
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Citation
Eva Hong, Sara Thulin Hedberg, Raquel Abad, Cecilia Fazio, Rocío Enríquez, et al.. Target gene sequencing to define the susceptibility of Neisseria meningitidis to ciprofloxacin.. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2013, 57 (4), pp.1961-1964. ⟨10.1128/AAC.02184-12⟩. ⟨pasteur-02089320⟩
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