Skip to Main content Skip to Navigation
Journal articles

Assessing Olfaction Using Ultrasonic Vocalization Recordings in Mouse Pups with a Sono-olfactometer

Abstract : [Abstract] Olfaction is the first sensory modality to develop during fetal life in mammals, and plays a key role in the various behaviors of neonates such as feeding and social interaction. Odorant cues (i.e., mother or predator scents) can trigger potentiation or inhibition of ultrasonic vocalizations (USV) emitted by pups following their isolation. Here, we report how USV are inhibited by olfactory cues using a sono-olfactometer that has been designed to quantify precisely olfaction in pups congenitally infected by cytomegalovirus. This olfactory-driven behavioral test assesses the USV emitted in presence of unfamiliar odorants such as citral scent or adult male mouse scent. We measure the number of USV emitted as an index of odorant detection during the three periods of the 5-min isolation time of the pup into the sono-olfactometer: first period without any odorant, second period with odorant exposure and last period with exhaust odorant. This protocol can be easily used to reveal olfactory deficits in pups with altered olfactory system due to toxic lesions or infectious diseases.
Document type :
Journal articles
Complete list of metadatas

Cited literature [28 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02028871
Contributor : Françoise Lazarini <>
Submitted on : Wednesday, February 20, 2019 - 10:21:56 AM
Last modification on : Monday, January 13, 2020 - 5:08:18 PM

File

Wagner Bio-protocol3170.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Sébastien Wagner, Pierre-Marie Lledo, Françoise Lazarini. Assessing Olfaction Using Ultrasonic Vocalization Recordings in Mouse Pups with a Sono-olfactometer. Bio-protocol , Bio-protocol LCC, 2019, 9 (4), ⟨10.21769/BioProtoc.3170⟩. ⟨pasteur-02028871⟩

Share

Metrics

Record views

78

Files downloads

200