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Journal articles
Facile immobilization of DNA using an enzymatic his-tag mimic
Pascal Röthlisberger
1
Fabienne Levi-Acobas
1
Ivo Sarac
1
Bruno Baron
2
Patrick England
2
Philippe Marlière
3
Piet Herdewijn
4
Marcel Hollenstein
1, *
Pascal Röthlisberger 1
Author
Fabienne Levi-Acobas 1
Author IdHAL : fabienne-levi-acobas ORCID : https://orcid.org/0000-0001-5022-2873
Ivo Sarac 1
Author
Bruno Baron 2
Author
Patrick England 2
Author IdHAL : patrick-england ORCID : https://orcid.org/0000-0001-6410-5918
Philippe Marlière 3
Author
Piet Herdewijn 4
Author
Marcel Hollenstein 1
Correspondent author IdHAL : marcel-hollenstein ResearcherId : http://www.researcherid.com/rid/O-6399-2014
1
Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids (Département de Biologie structurale et Chimie, 25-28, rue du Docteur Roux, 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 3523 (France)
2
Biophysique des macromolécules et leurs interactions (25-28 rue du Docteur Roux F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR3528 (France)
3
UEVE - Université d'Évry-Val-d'Essonne (Boulevard François Mitterrand 91025 Evry Cedex - France)
4
Rega Institute for Medical Research [Leuven, België] (Minderbroedersstraat 10, B 3000 Leuven - Belgium)
- KU Leuven - Catholic University of Leuven - Katholieke Universiteit Leuven (Oude Markt 13 - bus 5005, 3000 Leuven - Belgium)
Abstract : Methods for immobilization of DNA on solid supports are in high demand. Herein, we present a generally applicable enzymatic method for the immobilization of DNA without any prior chemical derivatization. This strategy relies on the homopolymerization of the modified triphosphate dImTP by the TdT. The resulting enzymatic his-tag mimic ensures binding of DNA on Ni-NTA agarose. The usefulness of this method is highlighted by the immobilization of functional nucleic acids without impairing their specific activities.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02012223
Contributor : Marcel Hollenstein Connect in order to contact the contributor
Submitted on : Friday, February 8, 2019 - 3:32:13 PM
Last modification on : Wednesday, June 17, 2020 - 6:18:02 PM
Contributor : Marcel Hollenstein Connect in order to contact the contributor
Submitted on : Friday, February 8, 2019 - 3:32:13 PM
Last modification on : Wednesday, June 17, 2020 - 6:18:02 PM
Identifiers
- HAL Id : pasteur-02012223, version 1
- DOI : 10.1039/c7cc07207d
- PUBMED : 29164188
Citation
Pascal Röthlisberger, Fabienne Levi-Acobas, Ivo Sarac, Bruno Baron, Patrick England, et al.. Facile immobilization of DNA using an enzymatic his-tag mimic. Chemical Communications, Royal Society of Chemistry, 2017, 53 (97), pp.13031-13034. ⟨10.1039/c7cc07207d⟩. ⟨pasteur-02012223⟩
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