Facile immobilization of DNA using an enzymatic his-tag mimic

Pascal Röthlisberger (1) , Fabienne Levi-Acobas (1) , Ivo Sarac (1) , Bruno Baron (2) , Patrick England (2) , Philippe Marlière (3) , Piet Herdewijn (4) , Marcel Hollenstein (1)

1 Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids
2 Biophysique des macromolécules et leurs interactions
3 UEVE - Université d'Évry-Val-d'Essonne
4 Rega Institute for Medical Research [Leuven, België]

Methods for immobilization of DNA on solid supports are in high demand. Herein, we present a generally applicable enzymatic method for the immobilization of DNA without any prior chemical derivatization. This strategy relies on the homopolymerization of the modified triphosphate dImTP by the TdT. The resulting enzymatic his-tag mimic ensures binding of DNA on Ni-NTA agarose. The usefulness of this method is highlighted by the immobilization of functional nucleic acids without impairing their specific activities.

Domains: Chemical Sciences; Organic chemistry

Pascal Röthlisberger, Fabienne Levi-Acobas, Ivo Sarac, Bruno Baron, Patrick England, et al.. Facile immobilization of DNA using an enzymatic his-tag mimic. Chemical Communications, 2017, 53 (97), pp.13031-13034. ⟨10.1039/c7cc07207d⟩. ⟨pasteur-02012223⟩