Facile immobilization of DNA using an enzymatic his-tag mimic

Pascal Röthlisberger; Fabienne Levi-Acobas; Ivo Sarac; Bruno Baron; Patrick England; Philippe Marlière; Piet Herdewijn; Marcel Hollenstein

doi:10.1039/c7cc07207d

Journal articles

Facile immobilization of DNA using an enzymatic his-tag mimic

Pascal Röthlisberger ¹ Fabienne Levi-Acobas ¹ Ivo Sarac ¹ Bruno Baron ² Patrick England ² Philippe Marlière ³ Piet Herdewijn ⁴ Marcel Hollenstein ^{1, *}

* Corresponding author

1 Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids

2 Biophysique des macromolécules et leurs interactions

3 UEVE - Université d'Évry-Val-d'Essonne

4 Rega Institute for Medical Research [Leuven, België]

Abstract : Methods for immobilization of DNA on solid supports are in high demand. Herein, we present a generally applicable enzymatic method for the immobilization of DNA without any prior chemical derivatization. This strategy relies on the homopolymerization of the modified triphosphate dImTP by the TdT. The resulting enzymatic his-tag mimic ensures binding of DNA on Ni-NTA agarose. The usefulness of this method is highlighted by the immobilization of functional nucleic acids without impairing their specific activities.

Document type :

Journal articles

Domain :

Chemical Sciences / Organic chemistry

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https://hal-pasteur.archives-ouvertes.fr/pasteur-02012223
Contributor : Marcel Hollenstein Connect in order to contact the contributor
Submitted on : Friday, February 8, 2019 - 3:32:13 PM
Last modification on : Wednesday, December 1, 2021 - 4:42:02 PM

Facile immobilization of DNA using an enzymatic his-tag mimic

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Facile immobilization of DNA using an enzymatic his-tag mimic

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