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Facile immobilization of DNA using an enzymatic his-tag mimic

Abstract : Methods for immobilization of DNA on solid supports are in high demand. Herein, we present a generally applicable enzymatic method for the immobilization of DNA without any prior chemical derivatization. This strategy relies on the homopolymerization of the modified triphosphate dImTP by the TdT. The resulting enzymatic his-tag mimic ensures binding of DNA on Ni-NTA agarose. The usefulness of this method is highlighted by the immobilization of functional nucleic acids without impairing their specific activities.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02012223
Contributor : Marcel Hollenstein <>
Submitted on : Friday, February 8, 2019 - 3:32:13 PM
Last modification on : Wednesday, June 17, 2020 - 6:18:02 PM

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Pascal Röthlisberger, Fabienne Levi-Acobas, Ivo Sarac, Bruno Baron, Patrick England, et al.. Facile immobilization of DNA using an enzymatic his-tag mimic. Chemical Communications, Royal Society of Chemistry, 2017, 53 (97), pp.13031-13034. ⟨10.1039/c7cc07207d⟩. ⟨pasteur-02012223⟩

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