Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids

Ivo Sarac (1) , Marcel Hollenstein (1)

1 Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids

The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template-independent addition of random nucleotides on 3'-overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide-based tools and methods, especially if combined with modified nucleoside triphosphates. Herein, we summarize the different applications stemming from the incorporation of modified nucleotides by the TdT. The structural, mechanistic, and biochemical properties of this polymerase are also discussed.

Keywords: DNA; end labeling; immobilization; nucleotides; terminal deoxynucleotidyl transferase

Domains: Chemical Sciences; Chemical Sciences; Organic chemistry

Ivo Sarac, Marcel Hollenstein. Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids. ChemBioChem, 2019, 20 (7), pp.860-871. ⟨10.1002/cbic.201800658⟩. ⟨pasteur-02012218⟩